Agilent Technologies G1978B setup guide 20 1 pk-pk 10 1 pk-pk

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Verification 2

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes

Table 2 6130 and 6140 Quad Performance Verification Summary, SIM Mode

 

G1978B

G1978B

 

ES Positive SIM Mode

APCI Positive SIM Mode

 

 

 

Solvent

75:25

75:25

 

methanol/water

methanol/water

 

with 5mM

with 5mM

 

ammonium formate

ammonium formate

 

 

 

Method name

6130SIMES_MM.M

6130SIMCI_MM.M

 

(6130 model)

(6140 model)

 

6140SIMES_MM.M

6140SIMCI_MM.M

 

(6140 model)

(6140 model)

 

 

 

Performance Specification

20: 1 pk-pk

10: 1 pk-pk

 

100: 1 rms

50: 1 rms

 

 

 

Sensitivity Verification for Multimode in ESI, Positive SIM Mode

1Start up the ChemStation software.

2Change the view to MSD Tune, and start an Autotune.

After the autotune completes, you may need to wait up to 30 minutes to allow for the calibrant solution to be pumped out of the Quad instrument. This minimizes any background signal resulting from the calibrant.

3Change the view to Method and Run Control, and load the method 6130SIMES_MM.M (6130 model) or 6140SIMES_MM.M (6140 model).

4Edit the method to ensure that 75:25 methanol/water with 5mM ammonium formate is selected as the LC solvent.

5Place the vials into the LC autosampler.

•Position #1: empty, uncapped vial

•Position #2: vial of the solvent used for dilution (solvent blank)

•Position #3: vial with the reserpine sample (1 pg/µL)

6Run the method.

The method does an FIA run with one injection of the empty vial, five injections of the solvent blank, and five injections of the reserpine sample.

You may need to further optimize the nebulizer pressure by running FIA

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide

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Contents Set-Up Guide U T I O N WA R N I N G This Guide Page Content Contents Installation Prepare to install InstallationFrom left to right G1946-80058, G1960-60802 and G1960-60856 Install the HV control PCA and cablesConnecting the valve PCA to the multimode HV power supply Connecting the Vcap and Vchamber cables to the power supply Connecting the HV PCA to the chassis To remove the multimode source Changing SourcesTo convert from multimode to ESI, Apci or Appi To convert from ESI, Apci or Appi to the multimode source Installation onStandard spray shield and capillary cap for ESI or Apci T E Multimode Spray Chamber No nebulizer on top of the multimode source LC/MS sample tubing connected to LC/MS inlet filter Verification Preparation 6110 and 6120 Quad Performance Verification Summary Table 6140 Quad Performance Verification Summary, SIM Mode 20 1 pk-pk 10 1 pk-pk Verification 6140 Quad Performance Verification Summary, Scan Mode 6130SCNESMM.M 6130SCNCIMM.M Verification Create and run a Multiple FIA Method Sequence Verification Autotune report To do an autotuneExample of multimode verification report Example of multimode verification report Methods Be impaired and the system may catch fire To setup a method to use the multimode sourceMethods Polarity options for Signal 1 and Signal Method Spray Chamber set to MM-ES+APCI To create a method for alternating ESI and Apci operationIonization mode for Signal 1 and Signal Index Index Page G1978-90070

G1978B specifications

Agilent Technologies G1978B is a high-performance mass spectrometer that is widely recognized for its innovations in analytical chemistry. This instrument stands out due to its combination of advanced technologies that enhance sensitivity, accuracy, and precision for complex analyses.

One of the key features of the G1978B is its compatibility with various ionization techniques, including Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI). This versatility allows users to analyze a diverse range of sample types, from small molecules to large biomolecules. Each ionization technique has its unique advantages, making the G1978B an excellent choice for laboratories focusing on pharmaceuticals, environmental analysis, and proteomics.

The G1978B is equipped with a sophisticated quadrupole mass filter, ensuring high-resolution mass measurements. This technology provides excellent mass accuracy and reproducibility, enabling users to distinguish between closely related compounds with minimal interference. The advanced data acquisition systems incorporated in the G1978B allow for rapid scanning capabilities, making it particularly effective for high-throughput screening applications.

In addition, the instrument features an intuitive user interface that simplifies method development and operational tasks. This includes streamlined software solutions that facilitate data analysis and interpretation, ensuring that even complex datasets can be managed efficiently. Furthermore, built-in diagnostic tools help in monitoring instrument performance, significantly reducing downtime and enhancing productivity.

The G1978B also incorporates advanced technologies for enhanced sensitivity. Its innovative source design minimizes background noise while maximizing signal intensity, making it particularly valuable for the detection of trace-level contaminants or low-abundance metabolites. Coupled with its exceptional dynamic range, the G1978B can quantify analytes over several orders of magnitude, which is crucial for applications in toxicology and clinical research.

With a robust design and ease of integration into existing laboratory workflows, the Agilent G1978B provides an ideal solution for researchers seeking a reliable mass spectrometry platform. Its combination of advanced ionization techniques, high-resolution capabilities, and user-friendly software makes it a versatile tool in modern analytical laboratories, paving the way for breakthroughs in research and development across a multitude of scientific disciplines.
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