Verification 2
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes
To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes
Preparation
1Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric flask. Use a clean graduated pipette.
2Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium formate.
3Transfer 5 mL of the first dilution to a 50 mL volumetric flask. Use a clean graduated pipette.
4Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium formate.
This provides the final 10 pg/µL reserpine concentration required for performance verification.
5Transfer approximately 1 mL of the second dilution to an autosampler vial.
Table 3 | 6130 and 6140 Quad Performance Verification Summary, Scan Mode | ||
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| G1978B | G1978B |
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| ES Positive Scan Mode | APCI Positive Scan Mode |
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Sample |
| Reserpine, 5 ng/µL | Reserpine, 5 ng/µL |
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Concentration after dilution | 10 pg/µL | 10 pg/µL | |
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Injection volume | 5 µL | 5 µL | |
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Total sample amount injected | 50 pg | 50 pg | |
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Sample order number | G2423A | G2423A | |
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Solvent |
| 75:25 | 75:25 |
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| methanol/water | methanol/water |
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| with 5mM | with 5mM |
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| ammonium formate | ammonium formate |
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Multimode Source for 6100 Series Single Quad LC/MS | 27 |