Agilent Technologies G1978B setup guide Verification

Verification 2

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes

When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The blank-subtracted average signal-to-noise value must be greater than or equal to 20:1 peak-to-peak (100:1 RMS).

Sensitivity Verification for Multimode in APCI, Positive Scan Mode

1Start the ChemStation software.

2Change the view to MSD Tune, and start an Autotune.

After the autotune completes, you may need to wait up to 30 minutes to allow for the calibrant solution to be pumped out of the Quad instrument. This minimizes any background signal resulting from the calibrant.

3Change the view to Method and Run Control, and load the method 6130SCNCI_MM.M (6130 model) or 6140SCNCI_MM.M (6140 model).

4Edit the method to ensure that 75:25 methanol/water with 5mM ammonium formate is selected as the LC solvent.

5Place the vials into the LC autosampler.

•Position #1: empty, uncapped vial

•Position #2: vial of the solvent used for dilution (solvent blank)

•Position #3: vial with the reserpine sample (10 pg/µL)

6Run the method.

The method does an FIA run with one injection of the empty vial, five injections of the solvent blank, and five injections of the reserpine sample.

You may need to further optimize the nebulizer pressure by running FIA experiments to achieve maximum instrument sensitivity

7Review the results.

When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The blank-subtracted average signal-to-noise value must be greater than or equal to 10:1 peak-to-peak (50:1 RMS).

Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 29