Oil Immersion Technique (cont.)
5.Slowly move the specimen stage upwards using the coarse adjustment knob until the oil drop on the slide comes into direct contact with the lens of the oil immersion objective.
6.Hold the knurled ring on the objective turret and swing the objective back and forth to eliminate air bubbles. Then bring the oil objective into the final position, so that the oil drop is between the front lens of the objective and the specimen slide.
7.Look through the microscope and slowly rotate the fine adjustment knob, so that the specimen stage moves upwards until the specimen is in focus.
8.If you are finished working with the oil immersion objective, clean the front side of the objective, the specimen slide, and all other surfaces that have come into contact with the oil, following the instructions in the "Care of the Microscope" section on page 46.
Leica DM750 | Manual | 41 |
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