Leica DM750 manual Troubleshooting, Make sure that a DF objective is being used

Page 49

Troubleshooting

Stand

The microscope does not respond.

Make sure that the socket has voltage.

Check the cable connections.

Make sure that the stand is connected to the power supply.

Check whether the fuse is defective and replace it if necessary (see page 16).

Focus

The specimen cannot be brought into focus.

Use the correct immersion medium.

Lay the specimen with the cover glass toward the top.

Make sure that the cover slip thickness is correct and that it meets the specifica- tions on the objective.

Dark field

 

Make sure that a DF objective is being used.

 

The objective aperture is too high (maximum 0.75/ 1.10); if necessary, reduce

No definite DF contrast is possible.

the objective aperture through the iris diaphragm on the objective.

 

Check the condenser centering.

 

Open the aperture diaphragm completely.

The image is not uniformly illuminated.

The objective magnification is too weak. Use a higher magnification.

Unwanted stray light.

Clean the specimen and neighboring lens surfaces.

Polarization

 

Leica DM750

Manual

49

 

 

 

Image 49 Leica DM750 manual Troubleshooting, Make sure that a DF objective is being used
Contents Leica DM750 Manual Manufacturer information Responsible for contentsPublished in November 2010 by Chapter overview Contents Safety regulations Safety concept Important information Light bulbsProvide clarity Explanatory notesImportant Notes Safety instructionsDescription Contact addressIntended Purpose of the Microscope Instructions on use So in the instructions For safety and potential hazardsPlace of use FungusScope of this manual Rights to any warranty claimsTransport DisposalHealth risks Health risks and dangers of useDual or detachable eyecups LED Klasse 1 /LED 1 Laser ProductInformation for the person responsible for the instrument Information for the person responsible for the instrumentCleaning glass surfaces Cleaning coated parts and plastic partsϘϘ Remove tough dirt using alcohol or petro- leum ether Care instructionsAccessories Accessories, maintenance and repairMaintenance Repairs and service workElectrical data and ambient conditions Fuse ReplacementElectrical data EnvironmentLeica DM750 Result if used improperly IntroductionUnpacking Ready Substage Illumination Adjustable Condenser CenteringLeica DM750 is available with two Types of Substage Illumination. Identify ToolTools used Attaching the viewing TubesϘϘ Allen key Leica EZ viewing tube with integrated eyepieces Leica EZ Viewing Tube integrated eyepiecesEyepieces Continue with the Eyecups section on Tightening the captive thumbscrew againInsert the eyepieces into the tubes Leica Standard Viewing Tube separated eyepiecesOrientation Eyecups Installing Objectives section onOrder to help block out ambient room light Installing Objectives Installing ObjectivesObjective nosepiece Ser needs to be installed Installing the Substage CondenserSubstage Condenser Thus roughly centered Set Setting the Illumination Intensity Power CordTurning on the microscope Work SurfaceDelivery package or, for a Leica DM750 Complete Condenser CenteringWith Koehler illumination, tighten Knurled screws Using the Condenser Using the CondenserSlide grips hold the slide in place Prepare to View a Specimen SlideFocusing Adjust the eyetubes Viewing Tube AdjustmentStandard Viewing Tube with One or Two Focusing Eyepieces Leica EZ Viewing TubeOn and your adjustments will be minimal Condenser focusing knob Koehler ConfigurationKoehler Configuration Oil Immersion Technique Oil Immersion Technique Changing the status of the time delay shutoff Time Delay ShutoffOr Disabled and you will not see any blinking No changes in the intensity controlLeica DM750 Manual Ready! Set! Go Care of the Microscope Instructions for cleaning General maintenanceStorage and handling Illumination Mechanical MaintenanceTroubleshooting Make sure that a DF objective is being used TroubleshootingPhase contrast Dimensions Leica DM750 Manual Leica DM750 Manual Leica DM750 Manual