Objective Nosepiece, Parfocal work, Objective change | Leica M205 C

The Objective Nosepiece

Use

The objective nosepiece enables you to switch between two objectives using just one hand movement, for example between a 1× and a 2× planapochromat.

Parfocal work

With the new M series objectives, the parfo- cality is maintained even when objectives are changed, meaning that the specimen remains in sharp focus during the change.

Older Leica objectives can continue to be used, but without parfocality during the

objective change.

Technical constraints

• The objective nosepiece cannot be used together with the LED5000 MCI™ system illumination, as the objective nosepiece

can no longer be rotated.

Leica M series	Manual	71

Image 71

Leica M205 C, M205 A Objective Nosepiece, Parfocal work, Objective change, Technical constraints, Can no longer be rotated

Contents

Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Action Symbols usedImportant information Provide clarity Non-intended use Safety regulationsDescription Designated use Transport EC Declaration of Conformity DisposalLegal regulations Light sources safety instructions Mercury lamp During maintenance work on the supply unitLamp housing Supply unit Table of Contents Leica LED5000 MCI Leica LED5000 RL Microscope Carrier AX Objective NosepieceDifferent Types of Objectives IsoPro Motorized XY Stage 110 Care, Maintenance, Contact Persons 113Leica PSC Controller 109 Leica M Series Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Eyepieces Maximum CompatibilityObjectives Tubes On We Go Assembly Hex socket screwdriver, 3 mm Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Tools used Hex socket screwdriver, 4 mm Optics carrier Assembling the tube TubeNo tools required Preparations Magnification range EyepiecesTools used Preparation Remove the protective cap on the optics  bly and disassembly so that it does not Instructions onObjective Larly to the 2× planapochromatic objective Remove the transport anchor from the objective nosepiece Objective Nosepiece AssemblyAssembly Do not fall onto the stage plate Tighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Hex socket screwdriver, 2.5 mm Check the switch positionAX Carrier Preparation Tools Hex socket screwdriver, 4 mm Philips screwdriver AX Carrier Assembly Transmitted-light Base TL ST Unpacking the base Focusing drive and column Anchors as follows Removing the transport anchors Standard stage Stage assemblyTransmitted-light Base TL Bfdf Standard delivery TL RC / TL RCI Left or right operation IsoPro Manual Cross-stage AssemblyIsoPro Cross-stage Place the cross-stage on the base Control assemblyCross-stage assembly Focusing drive and column Basics IsoPro Motorized Cross-stage Assembly IsoPro Motorized Cross-stage Assembly Damaged Place the cross-stage on the base BasesCross-stage and base Cross-stage for X-Y Stage Control Module Terminals Cables TerminalsInterface to the optics carrier is on the column Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Stirrups on both top holes on the drive hous Ing Leica LED5000 MCIHold the LED5000 MCI with one Hand and tightly screw the retraining Be facing downwards Installation on the column Leica LED5000 MCI Alternative Assembly Column Leica LED5000 RLMust be facing upwards Into place Quick Start Guide Fastest Route to Success Overview of an M series Microscope Look into the eyepieces Adjusting the Interpupillary DistanceCorrect Interpupillary Distance Reference value If you wear glasses Using the EyepiecesDioptric correction If you do not wear glasses Coarse/fine adjustment FocusingFocusing drive can be operated either left- or right-handed Focusing Adjusting the resistance Adjusting the resistance of the focus drive Look into the eyepieces Focus on the specimen Changing the magnification zoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Enlarge the view to the maximum level Focus on the specimen Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Parfocality Opening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Leica partner Health NotesPotential sources of infection Dioptric correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Convergence angle in the stereoscopic image GraticulesUse Use with the AX carrier Photography & Video Leica Application Suite Photography & VideoAdapter Leica DFC cameras Photo tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100% Switchover Microscope Carrier Stereoscopic viewing Microscope Carrier AXCompatibility Coaxial light and the Leica M205 C Parallax-free images Technical constraints Objective NosepieceParfocal work Objective change Objectives Optical accessories Achromatic objectives with a long focal length Different Types of ObjectivesAchromatic objectives Planachromatic objective 1× Bases Transmitted-light Base TL ST Controls Bright field Transmitted-light Base TL ST OperationLight intensity control Transmitted-light control Changing the Halogen Lamp Transmitted-light Base TL ST Lamp ReplacementPrecautionary measures Transmitted-light Base TL Bfdf Controls Adapter at the focusing drive Turn the control as far as it will go towards DF dark field Transmitted-light Base TL Bfdf OperationLight source Dark field TL RC / TL RCI Controls Functions of the rotary knob TL RCI The Deflection MirrorInverted world? Tilted mirror Using a USB mouse only TL RCI TL RCI Color Intensity and Temperature TL RC / TL RCI Operation Setting the relief contrast Suitable for fixed specimens and fine struc- tures TL RCI Methods in Transmitted LightVertical bright-field illumination Single-sided dark field Negative relief contrast TL RCI Relief ImagesStarting position Positive relief contrast Suitable for semitransparent and transparent specimens Dynamic relief contrast Daylight filter for TL ST Using FiltersFilters for TL RC and TL RCI Operating the cross-stage IsoPro IsoPro Non-motorized Controls Stage DCI module IsoPro Motorized ControlsIsoPro System Illumination Antees the reproducibility of an experiment Use Contact with the baseBeneath the MCI Bottom end of the guide rods Maximum brightness Using the keyboardUse the key to switch the illumination on or off Illumination scenarios Constraint Accessories Fine control of the cross-stage Leica PSC ControllerConnection Passing over the specimen quickly Dimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Illumination types Ambient temperature 10-40 CConnections Power plug, power switch Weight Energy consumption 30 W max Concave mirror *** With external light source Internal shutter/lamp control Yes Integrated filter holder Concave mirror **** With external light source Active f=10 mm, end tube f=13 mm WeightLeica TL RC Leica TL RCI Compatibility IsoPro Motorized XY Stage Appendix Calculation example magnification in binocular tube ParameterExample Mail contact Care, Maintenance, Contact PersonsWarranty benefits Contact address Dust and dirt will affect the quality of your results Keep accessories in a dust-free place when not in useCleaning polymer components Protection from dirt