Dynamic relief contrast | Leica M205 C, M205 A, M165 C instruction

TL RCI™: Relief Images (continued)

Dynamic relief contrast

Suitable for semitransparent and transparent specimens.

By tilting the deflection mirror slightly, you can move the gaps over the entire field of view and quickly toggle between positive and negative relief images. The dynamic effect makes it easy to distinguish phase structures from amplitude structures.

Constraints

The relief methods provide good results from mid-zoom to high magnifications and with 1×, 1.6× and 2× magnifications. In the lower zoom half and with weaker specimens, the object field may not be uniformly illuminated.

We recommend using the transmitted-light base with 1× or higher objectives, and not objectives with a long focal length.

Leica M series	Manual	86

Image 86

Leica M205 C, M205 A, M165 C manual Dynamic relief contrast, Suitable for semitransparent and transparent specimens

Contents

Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Provide clarity Symbols usedImportant information Action Designated use Safety regulationsDescription Non-intended use Transport EC Declaration of Conformity DisposalLegal regulations Light sources safety instructions Supply unit During maintenance work on the supply unitLamp housing Mercury lamp Table of Contents Leica LED5000 MCI Leica LED5000 RL Microscope Carrier AX Objective NosepieceDifferent Types of Objectives 109 Care, Maintenance, Contact Persons 113Leica PSC Controller IsoPro Motorized XY Stage 110 Leica M Series Congratulations Step Towards Infinity Limits are made to be broken Reliability for your experiments Fewer cables Modular Design Everything is Relative Have a special request? Let us know Tubes Maximum CompatibilityObjectives Eyepieces On We Go Assembly Tools used Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Hex socket screwdriver, 3 mm Optics carrier Hex socket screwdriver, 4 mm Preparations TubeNo tools required Assembling the tube Magnification range EyepiecesTools used Preparation Larly to the 2× planapochromatic objective  bly and disassembly so that it does not Instructions onObjective Remove the protective cap on the optics Do not fall onto the stage plate Objective Nosepiece AssemblyAssembly Remove the transport anchor from the objective nosepiece Tighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Tools Check the switch positionAX Carrier Preparation Hex socket screwdriver, 2.5 mm AX Carrier Assembly Hex socket screwdriver, 4 mm Philips screwdriver Unpacking the base Focusing drive and column Transmitted-light Base TL ST Removing the transport anchors Anchors as follows Standard delivery Stage assemblyTransmitted-light Base TL Bfdf Standard stage TL RC / TL RCI Left or right operation IsoPro Manual Cross-stage AssemblyIsoPro Cross-stage Place the cross-stage on the base Control assemblyCross-stage assembly Focusing drive and column IsoPro Motorized Cross-stage Assembly Basics IsoPro Motorized Cross-stage Assembly Damaged Place the cross-stage on the base BasesCross-stage and base Cross-stage for X-Y Stage Control Module Terminals Cables TerminalsInterface to the optics carrier is on the column Cables Cable Duct Feeding the cables Cables Diagram Alternative installation Hand and tightly screw the retraining Be facing downwards Leica LED5000 MCIHold the LED5000 MCI with one Stirrups on both top holes on the drive hous Ing Leica LED5000 MCI Alternative Assembly Installation on the column Into place Leica LED5000 RLMust be facing upwards Column Quick Start Guide Fastest Route to Success Overview of an M series Microscope Reference value Adjusting the Interpupillary DistanceCorrect Interpupillary Distance Look into the eyepieces If you do not wear glasses Using the EyepiecesDioptric correction If you wear glasses Focusing FocusingFocusing drive can be operated either left- or right-handed Coarse/fine adjustment Adjusting the resistance of the focus drive Adjusting the resistance Look into the eyepieces Focus on the specimen Changing the magnification zoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Parfocality Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Enlarge the view to the maximum level Focus on the specimen Opening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M series Leica partner Health NotesPotential sources of infection Using the Dioptric Correction Dioptric correction Adjusting Dioptric Correction and Parfocality Use with the AX carrier GraticulesUse Convergence angle in the stereoscopic image Photography & Video Leica DFC cameras Photography & VideoAdapter Leica Application Suite Photo tubes and C-mounts Trinocular video/phototube 50% Switchover Trinocular video/phototube 100% Microscope Carrier Stereoscopic viewing Microscope Carrier AXCompatibility Parallax-free images Coaxial light and the Leica M205 C Objective change Objective NosepieceParfocal work Technical constraints Objectives Optical accessories Planachromatic objective 1× Different Types of ObjectivesAchromatic objectives Achromatic objectives with a long focal length Bases Transmitted-light Base TL ST Controls Transmitted-light control Transmitted-light Base TL ST OperationLight intensity control Bright field Changing the Halogen Lamp Transmitted-light Base TL ST Lamp ReplacementPrecautionary measures Adapter at the focusing drive Transmitted-light Base TL Bfdf Controls Dark field Transmitted-light Base TL Bfdf OperationLight source Turn the control as far as it will go towards DF dark field TL RC / TL RCI Controls Tilted mirror TL RCI The Deflection MirrorInverted world? Functions of the rotary knob TL RCI Color Intensity and Temperature Using a USB mouse only TL RCI Setting the relief contrast TL RC / TL RCI Operation Single-sided dark field TL RCI Methods in Transmitted LightVertical bright-field illumination Suitable for fixed specimens and fine struc- tures Positive relief contrast TL RCI Relief ImagesStarting position Negative relief contrast Dynamic relief contrast Suitable for semitransparent and transparent specimens Daylight filter for TL ST Using FiltersFilters for TL RC and TL RCI IsoPro Non-motorized Controls Operating the cross-stage IsoPro Stage DCI module IsoPro Motorized ControlsIsoPro System Illumination Bottom end of the guide rods Use Contact with the baseBeneath the MCI Antees the reproducibility of an experiment Illumination scenarios Using the keyboardUse the key to switch the illumination on or off Maximum brightness Constraint Accessories Passing over the specimen quickly Leica PSC ControllerConnection Fine control of the cross-stage Dimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Energy consumption 30 W max Ambient temperature 10-40 CConnections Power plug, power switch Weight Illumination types Internal shutter/lamp control Yes Integrated filter holder Concave mirror *** With external light source Concave mirror **** With external light source Active f=10 mm, end tube f=13 mm WeightLeica TL RC Leica TL RCI IsoPro Motorized XY Stage Compatibility Appendix Calculation example magnification in binocular tube ParameterExample Contact address Care, Maintenance, Contact PersonsWarranty benefits Mail contact Protection from dirt Keep accessories in a dust-free place when not in useCleaning polymer components Dust and dirt will affect the quality of your results