TL RCI Relief Images, Starting position | Leica M165 C, M205 A

TL RCI™: Relief Images

Starting position

1.Push the deflection mirror all the way back towards the column.

2.Turn the deflection mirror into the notch position at an angle of 45°.

Positive relief contrast

Suitable for semitransparent and transparent specimens. The phase structures look like hills.

The effect can be strengthened or weakened by gently tilting the deflection mirror.

Negative relief contrast

Suitable for semitransparent and transparent specimens. These settings result in a nega- tive relief contrast. Phase structures look like valleys.

The effect can be strengthened or weakened by gently tilting the deflection mirror.

Leica M series	Manual	85

Image 85

Leica M165 C, M205 A, M205 C TL RCI Relief Images, Starting position, Positive relief contrast, Negative relief contrast

Contents

Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Important information Symbols usedProvide clarity Action Description Safety regulationsDesignated use Non-intended use Legal regulations DisposalTransport EC Declaration of Conformity Light sources safety instructions Lamp housing During maintenance work on the supply unitSupply unit Mercury lamp Table of Contents Different Types of Objectives Microscope Carrier AX Objective NosepieceLeica LED5000 MCI Leica LED5000 RL Leica PSC Controller Care, Maintenance, Contact Persons 113109 IsoPro Motorized XY Stage 110 Leica M Series Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Objectives Maximum CompatibilityTubes Eyepieces On We Go Assembly Assembling the column adapter Assembling the focusing drive Base and Focusing ColumnTools used Hex socket screwdriver, 3 mm Hex socket screwdriver, 4 mm Optics carrier No tools required TubePreparations Assembling the tube Tools used Preparation EyepiecesMagnification range Objective  bly and disassembly so that it does not Instructions onLarly to the 2× planapochromatic objective Remove the protective cap on the optics Assembly Objective Nosepiece AssemblyDo not fall onto the stage plate Remove the transport anchor from the objective nosepiece Adjustment Objective Nosepiece Adjusting ParfocalityTighten the locking screws AX Carrier Preparation Check the switch positionTools Hex socket screwdriver, 2.5 mm Hex socket screwdriver, 4 mm Philips screwdriver AX Carrier Assembly Transmitted-light Base TL ST Unpacking the base Focusing drive and column Anchors as follows Removing the transport anchors Transmitted-light Base TL Bfdf Stage assemblyStandard delivery Standard stage TL RC / TL RCI IsoPro Cross-stage IsoPro Manual Cross-stage AssemblyLeft or right operation Cross-stage assembly Control assemblyPlace the cross-stage on the base Focusing drive and column Basics IsoPro Motorized Cross-stage Assembly IsoPro Motorized Cross-stage Assembly Cross-stage and base BasesDamaged Place the cross-stage on the base Cross-stage for X-Y Stage Control Module Interface to the optics carrier is on the column Cables TerminalsTerminals Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Hold the LED5000 MCI with one Leica LED5000 MCIHand and tightly screw the retraining Be facing downwards Stirrups on both top holes on the drive hous Ing Installation on the column Leica LED5000 MCI Alternative Assembly Must be facing upwards Leica LED5000 RLInto place Column Quick Start Guide Fastest Route to Success Overview of an M series Microscope Correct Interpupillary Distance Adjusting the Interpupillary DistanceReference value Look into the eyepieces Dioptric correction Using the EyepiecesIf you do not wear glasses If you wear glasses Focusing drive can be operated either left- or right-handed FocusingFocusing Coarse/fine adjustment Adjusting the resistance Adjusting the resistance of the focus drive Zooming Changing the magnification zoomLook into the eyepieces Focus on the specimen Enabling and disabling ratchet steps Ratchet Steps and Magnification LevelsMagnifications and fields of view Requirements for parfocal work Parfocality More Comfort and Convenience for Your WorkParfocality Enlarge the view to the maximum level Focus on the specimen Closing the iris diaphragm Iris DiaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Potential sources of infection Health NotesLeica partner Dioptric correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Use GraticulesUse with the AX carrier Convergence angle in the stereoscopic image Photography & Video Adapter Photography & VideoLeica DFC cameras Leica Application Suite Photo tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100% Switchover Microscope Carrier Compatibility Microscope Carrier AXStereoscopic viewing Coaxial light and the Leica M205 C Parallax-free images Parfocal work Objective NosepieceObjective change Technical constraints Objectives Optical accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Transmitted-light Base TL ST Controls Light intensity control Transmitted-light Base TL ST OperationTransmitted-light control Bright field Precautionary measures Transmitted-light Base TL ST Lamp ReplacementChanging the Halogen Lamp Transmitted-light Base TL Bfdf Controls Adapter at the focusing drive Light source Transmitted-light Base TL Bfdf OperationDark field Turn the control as far as it will go towards DF dark field TL RC / TL RCI Controls Inverted world? TL RCI The Deflection MirrorTilted mirror Functions of the rotary knob Using a USB mouse only TL RCI TL RCI Color Intensity and Temperature TL RC / TL RCI Operation Setting the relief contrast Vertical bright-field illumination TL RCI Methods in Transmitted LightSingle-sided dark field Suitable for fixed specimens and fine struc- tures Starting position TL RCI Relief ImagesPositive relief contrast Negative relief contrast Suitable for semitransparent and transparent specimens Dynamic relief contrast Filters for TL RC and TL RCI Using FiltersDaylight filter for TL ST Operating the cross-stage IsoPro IsoPro Non-motorized Controls IsoPro IsoPro Motorized ControlsStage DCI module System Illumination Beneath the MCI Use Contact with the baseBottom end of the guide rods Antees the reproducibility of an experiment Use the key to switch the illumination on or off Using the keyboardIllumination scenarios Maximum brightness Constraint Accessories Connection Leica PSC ControllerPassing over the specimen quickly Fine control of the cross-stage Dimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Connections Power plug, power switch Weight Ambient temperature 10-40 CEnergy consumption 30 W max Illumination types Concave mirror *** With external light source Internal shutter/lamp control Yes Integrated filter holder Leica TL RC Leica TL RCI Active f=10 mm, end tube f=13 mm WeightConcave mirror **** With external light source Compatibility IsoPro Motorized XY Stage Appendix Example ParameterCalculation example magnification in binocular tube Warranty benefits Care, Maintenance, Contact PersonsContact address Mail contact Cleaning polymer components Keep accessories in a dust-free place when not in useProtection from dirt Dust and dirt will affect the quality of your results