TL RCI Methods in Transmitted Light | Leica M205 A, M165 C, M205 C

TL RCI™: Methods in Transmitted Light

Vertical bright-field illumination

Suitable for stained amplitude specimens with sufficient contrast.

The light beams are deflected vertically through the specimen. This results in an accurate bright field with maximum brightness.

Inclined transmitted light

Suitable for semitransparent, opaque speci- mens such as foraminifera and fish eggs. Move the deflection mirror until the desired data are visible.

Single-sided dark field

Suitable for fixed specimens and fine struc- tures.

The flatter the angle at which the light beams are deflected into the specimen plane, the darker the substrate appears. A dark field-like transmitted light is created. Outlines, fine edges and structures are bright, in contrast with the dark background, through diffraction of the light beams on the dark background.

Leica M series	Manual	84

Image 84

Leica M205 A, M165 C manual TL RCI Methods in Transmitted Light, Vertical bright-field illumination, Single-sided dark field

Contents

Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Symbols used Important informationProvide clarity Action Safety regulations DescriptionDesignated use Non-intended use Disposal Legal regulationsTransport EC Declaration of Conformity Light sources safety instructions During maintenance work on the supply unit Lamp housingSupply unit Mercury lamp Table of Contents Microscope Carrier AX Objective Nosepiece Different Types of ObjectivesLeica LED5000 MCI Leica LED5000 RL Care, Maintenance, Contact Persons 113 Leica PSC Controller109 IsoPro Motorized XY Stage 110 Leica M Series Congratulations Step Towards Infinity Limits are made to be broken Reliability for your experiments Fewer cables Modular Design Everything is Relative Have a special request? Let us know Maximum Compatibility ObjectivesTubes Eyepieces On We Go Assembly Base and Focusing Column Assembling the column adapter Assembling the focusing driveTools used Hex socket screwdriver, 3 mm Optics carrier Hex socket screwdriver, 4 mm Tube No tools requiredPreparations Assembling the tube Eyepieces Tools used PreparationMagnification range  bly and disassembly so that it does not Instructions on ObjectiveLarly to the 2× planapochromatic objective Remove the protective cap on the optics Objective Nosepiece Assembly AssemblyDo not fall onto the stage plate Remove the transport anchor from the objective nosepiece Objective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws Check the switch position AX Carrier PreparationTools Hex socket screwdriver, 2.5 mm AX Carrier Assembly Hex socket screwdriver, 4 mm Philips screwdriver Unpacking the base Focusing drive and column Transmitted-light Base TL ST Removing the transport anchors Anchors as follows Stage assembly Transmitted-light Base TL BfdfStandard delivery Standard stage TL RC / TL RCI IsoPro Manual Cross-stage Assembly IsoPro Cross-stageLeft or right operation Control assembly Cross-stage assemblyPlace the cross-stage on the base Focusing drive and column IsoPro Motorized Cross-stage Assembly Basics IsoPro Motorized Cross-stage Assembly Bases Cross-stage and baseDamaged Place the cross-stage on the base Cross-stage for X-Y Stage Control Module Cables Terminals Interface to the optics carrier is on the columnTerminals Cables Cable Duct Feeding the cables Cables Diagram Alternative installation Leica LED5000 MCI Hold the LED5000 MCI with oneHand and tightly screw the retraining Be facing downwards Stirrups on both top holes on the drive hous Ing Leica LED5000 MCI Alternative Assembly Installation on the column Leica LED5000 RL Must be facing upwardsInto place Column Quick Start Guide Fastest Route to Success Overview of an M series Microscope Adjusting the Interpupillary Distance Correct Interpupillary DistanceReference value Look into the eyepieces Using the Eyepieces Dioptric correctionIf you do not wear glasses If you wear glasses Focusing Focusing drive can be operated either left- or right-handedFocusing Coarse/fine adjustment Adjusting the resistance of the focus drive Adjusting the resistance Changing the magnification zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Parfocality More Comfort and Convenience for Your Work Requirements for parfocal workParfocality Enlarge the view to the maximum level Focus on the specimen Iris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M series Health Notes Potential sources of infectionLeica partner Using the Dioptric Correction Dioptric correction Adjusting Dioptric Correction and Parfocality Graticules UseUse with the AX carrier Convergence angle in the stereoscopic image Photography & Video Photography & Video AdapterLeica DFC cameras Leica Application Suite Photo tubes and C-mounts Trinocular video/phototube 50% Switchover Trinocular video/phototube 100% Microscope Carrier Microscope Carrier AX CompatibilityStereoscopic viewing Parallax-free images Coaxial light and the Leica M205 C Objective Nosepiece Parfocal workObjective change Technical constraints Objectives Optical accessories Different Types of Objectives Achromatic objectivesPlanachromatic objective 1× Achromatic objectives with a long focal length Bases Transmitted-light Base TL ST Controls Transmitted-light Base TL ST Operation Light intensity controlTransmitted-light control Bright field Transmitted-light Base TL ST Lamp Replacement Precautionary measuresChanging the Halogen Lamp Adapter at the focusing drive Transmitted-light Base TL Bfdf Controls Transmitted-light Base TL Bfdf Operation Light sourceDark field Turn the control as far as it will go towards DF dark field TL RC / TL RCI Controls TL RCI The Deflection Mirror Inverted world?Tilted mirror Functions of the rotary knob TL RCI Color Intensity and Temperature Using a USB mouse only TL RCI Setting the relief contrast TL RC / TL RCI Operation TL RCI Methods in Transmitted Light Vertical bright-field illuminationSingle-sided dark field Suitable for fixed specimens and fine struc- tures TL RCI Relief Images Starting positionPositive relief contrast Negative relief contrast Dynamic relief contrast Suitable for semitransparent and transparent specimens Using Filters Filters for TL RC and TL RCIDaylight filter for TL ST IsoPro Non-motorized Controls Operating the cross-stage IsoPro IsoPro Motorized Controls IsoProStage DCI module System Illumination Use Contact with the base Beneath the MCIBottom end of the guide rods Antees the reproducibility of an experiment Using the keyboard Use the key to switch the illumination on or offIllumination scenarios Maximum brightness Constraint Accessories Leica PSC Controller ConnectionPassing over the specimen quickly Fine control of the cross-stage Dimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Ambient temperature 10-40 C Connections Power plug, power switch WeightEnergy consumption 30 W max Illumination types Internal shutter/lamp control Yes Integrated filter holder Concave mirror *** With external light source Active f=10 mm, end tube f=13 mm Weight Leica TL RC Leica TL RCIConcave mirror **** With external light source IsoPro Motorized XY Stage Compatibility Appendix Parameter ExampleCalculation example magnification in binocular tube Care, Maintenance, Contact Persons Warranty benefitsContact address Mail contact Keep accessories in a dust-free place when not in use Cleaning polymer componentsProtection from dirt Dust and dirt will affect the quality of your results