Transmitted-light Base TL Bfdf Operation, Dark field | Leica M165 C

Transmitted-light Base TL BFDF: Operation

Light intensity control

• Please observe the user manual—in particular, all safety regulations—from the manufacturers of the light guide and cold

light source.

★★ Switch on the cold light source according to the manufacturer's user manual and adjust the brightness.

Transmitted-light control

The TL BFDF transmitted-light base has a poten- tiometer that switches the light from "bright field" to "dark field".

Bright field

Bright field is suitable for examining translu- cent objects featuring contrasting structures. The object is directly illuminated from below and is seen in its natural colors against a bright background.

★★ Turn the control as far as it will go towards "BF" ("bright field").

Dark field

In dark-field illumination, a ring illuminator is used in such a way that the direct light

does not reach the objective without a speci- men. Only the structure of semitransparent, opaque objects disperses the light, making the object visible against a dark background.

★★ Turn the control as far as it will go towards "DF" ("dark field").

Fingertip with bright field illumination

Identical subject with dark field illumination

Leica M series	Manual	79

Image 79

Leica M165 C, M205 A, M205 C manual Transmitted-light Base TL Bfdf Operation, Light source, Dark field

Contents

Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Action Symbols usedImportant information Provide clarity Non-intended use Safety regulationsDescription Designated use Legal regulations DisposalTransport EC Declaration of Conformity Light sources safety instructions Mercury lamp During maintenance work on the supply unitLamp housing Supply unit Table of Contents Different Types of Objectives Microscope Carrier AX Objective NosepieceLeica LED5000 MCI Leica LED5000 RL IsoPro Motorized XY Stage 110 Care, Maintenance, Contact Persons 113Leica PSC Controller 109 Leica M Series Congratulations Limits are made to be broken Step Towards Infinity Fewer cables Reliability for your experiments Have a special request? Let us know Modular Design Everything is Relative Eyepieces Maximum CompatibilityObjectives Tubes On We Go Assembly Hex socket screwdriver, 3 mm Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Tools used Hex socket screwdriver, 4 mm Optics carrier Assembling the tube TubeNo tools required Preparations Tools used Preparation EyepiecesMagnification range Remove the protective cap on the optics  bly and disassembly so that it does not Instructions onObjective Larly to the 2× planapochromatic objective Remove the transport anchor from the objective nosepiece Objective Nosepiece AssemblyAssembly Do not fall onto the stage plate Adjustment Objective Nosepiece Adjusting ParfocalityTighten the locking screws Hex socket screwdriver, 2.5 mm Check the switch positionAX Carrier Preparation Tools Hex socket screwdriver, 4 mm Philips screwdriver AX Carrier Assembly Transmitted-light Base TL ST Unpacking the base Focusing drive and column Anchors as follows Removing the transport anchors Standard stage Stage assemblyTransmitted-light Base TL Bfdf Standard delivery TL RC / TL RCI IsoPro Cross-stage IsoPro Manual Cross-stage AssemblyLeft or right operation Cross-stage assembly Control assemblyPlace the cross-stage on the base Focusing drive and column Basics IsoPro Motorized Cross-stage Assembly IsoPro Motorized Cross-stage Assembly Cross-stage and base BasesDamaged Place the cross-stage on the base Cross-stage for X-Y Stage Control Module Interface to the optics carrier is on the column Cables TerminalsTerminals Feeding the cables Cables Cable Duct Alternative installation Cables Diagram Stirrups on both top holes on the drive hous Ing Leica LED5000 MCIHold the LED5000 MCI with one Hand and tightly screw the retraining Be facing downwards Installation on the column Leica LED5000 MCI Alternative Assembly Column Leica LED5000 RLMust be facing upwards Into place Quick Start Guide Fastest Route to Success Overview of an M series Microscope Look into the eyepieces Adjusting the Interpupillary DistanceCorrect Interpupillary Distance Reference value If you wear glasses Using the EyepiecesDioptric correction If you do not wear glasses Coarse/fine adjustment FocusingFocusing drive can be operated either left- or right-handed Focusing Adjusting the resistance Adjusting the resistance of the focus drive Zooming Changing the magnification zoomLook into the eyepieces Focus on the specimen Enabling and disabling ratchet steps Ratchet Steps and Magnification LevelsMagnifications and fields of view Enlarge the view to the maximum level Focus on the specimen Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Parfocality Closing the iris diaphragm Iris DiaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the Eyepieces Potential sources of infection Health NotesLeica partner Dioptric correction Using the Dioptric Correction Dioptric Correction and Parfocality Adjusting Convergence angle in the stereoscopic image GraticulesUse Use with the AX carrier Photography & Video Leica Application Suite Photography & VideoAdapter Leica DFC cameras Photo tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100% Switchover Microscope Carrier Compatibility Microscope Carrier AXStereoscopic viewing Coaxial light and the Leica M205 C Parallax-free images Technical constraints Objective NosepieceParfocal work Objective change Objectives Optical accessories Achromatic objectives with a long focal length Different Types of ObjectivesAchromatic objectives Planachromatic objective 1× Bases Transmitted-light Base TL ST Controls Bright field Transmitted-light Base TL ST OperationLight intensity control Transmitted-light control Precautionary measures Transmitted-light Base TL ST Lamp ReplacementChanging the Halogen Lamp Transmitted-light Base TL Bfdf Controls Adapter at the focusing drive Turn the control as far as it will go towards DF dark field Transmitted-light Base TL Bfdf OperationLight source Dark field TL RC / TL RCI Controls Functions of the rotary knob TL RCI The Deflection MirrorInverted world? Tilted mirror Using a USB mouse only TL RCI TL RCI Color Intensity and Temperature TL RC / TL RCI Operation Setting the relief contrast Suitable for fixed specimens and fine struc- tures TL RCI Methods in Transmitted LightVertical bright-field illumination Single-sided dark field Negative relief contrast TL RCI Relief ImagesStarting position Positive relief contrast Suitable for semitransparent and transparent specimens Dynamic relief contrast Filters for TL RC and TL RCI Using FiltersDaylight filter for TL ST Operating the cross-stage IsoPro IsoPro Non-motorized Controls IsoPro IsoPro Motorized ControlsStage DCI module System Illumination Antees the reproducibility of an experiment Use Contact with the baseBeneath the MCI Bottom end of the guide rods Maximum brightness Using the keyboardUse the key to switch the illumination on or off Illumination scenarios Constraint Accessories Fine control of the cross-stage Leica PSC ControllerConnection Passing over the specimen quickly Dimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Illumination types Ambient temperature 10-40 CConnections Power plug, power switch Weight Energy consumption 30 W max Concave mirror *** With external light source Internal shutter/lamp control Yes Integrated filter holder Leica TL RC Leica TL RCI Active f=10 mm, end tube f=13 mm WeightConcave mirror **** With external light source Compatibility IsoPro Motorized XY Stage Appendix Example ParameterCalculation example magnification in binocular tube Mail contact Care, Maintenance, Contact PersonsWarranty benefits Contact address Dust and dirt will affect the quality of your results Keep accessories in a dust-free place when not in useCleaning polymer components Protection from dirt