Leica Leica VT1200 Toggling from Auto, Toggling from MAN to An audible warning signal sounds

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5. Working with the Instrument

Key/setting dial

semiautomatic sectioning mode

automatic sectioning mode

 

 

 

In semiautomatic sectioning mode, before each cut, a manual feed to the desired section thickness must be carried out using the setting dial for section thickness.

There is no automatic specimen retraction in this mode; however, retraction can be performed man- ually.

In automatic mode, the selected section thickness feed (AUTO FEED) is carried out along the 1st selected cutting window edge automatically before each cut. To prevent the specimen surface and the blade from coming into contact while the blade is being retracted, the specimen is lowered by the desired retraction value along the 2nd cutting window edge after each complete cut.

Toggling from AUTO

The following key functions are dis-

to MAN

abled in semiautomatic mode:

 

• Setting cutting window edges

 

• Selecting the continuous stroke

 

(CONT)

 

• Selecting the section thickness

 

for the automatic feed (AUTO

 

FEED)

 

• PAUSE

 

Toggling from MAN to

An audible warning signal sounds

The following functions keys be-

AUTO

when these keys are pressed.

come enabled again in automatic

 

 

mode:

 

 

• Cutting window edges that have

 

 

already been set

 

 

• Section thickness (AUTO FEED)

 

 

and continuous stroke (CONT)

Leica VT1200 and VT1200S – Microtome

23

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Contents Leica VT1200 Leica VT1200S Page Nussloch Germany Contents Qualification of Personnel Symbols in the Text and their MeaningsImportant Notes Intended Use/Improper UseGeneral Safety Notes SafetyProper handling Transport, Unpacking and Setting upElectrical data Instrument CharacteristicsTechnical Data for the VT1200 Ambient conditions Instrument CharacteristicsTechnical Data for the VT1200S DimensionsMicroscope General Overview of the VT1200/VT1200SBlades for the blade holder Attachments on dovetail receptacleLED for feed rate VT1200 Control PanelWindow edge is set Moves the specimen Upwards as long as 1 VT1200S Control PanelStandard Scope of Delivery for the VT1200 Installation91200S001 Standard Scope of Delivery for the VT1200S0481 Packing the instrument Packing and Setting up the InstrumentBefore Commissioning the Instrument Description of the Typical Application Working with the InstrumentMoving the Specimen Receptacle Vertically Control Elements on the VT1200 Control Panel Blade Forward back Blade MethodStarts the sectioning process Selecting the Sectioning ParametersSelected amplitude Ampl Saved section thickness LED illumination is switched onControl Element on the Control Panel of the VT1200S Blade feed rate SpeedMode Toggling from MAN to An audible warning signal soundsWhen these keys are pressed Toggling from AutoMm in 0.05 mm increments Not possible Adjusting the amplitude fromKey/setting dial Automatic mode max μmWorking with the Instrument Blade forward and Blade Ward key and press 2nd cutting Key lights upWard key. Press the 1st cutting Window edge key until the LEDProcess Μm display is then reset toAutomatic section thickness Or 100μm by max μm using rotary knobUsing predefined step sizes 1 With rotary knob 1. If continuous MAN with rotary knob 1 forStroke type CUT only single Stroke Single can be selected5 mm/s in 0.5 mm/s increments Installing the Ice Bath and Buffer Tray Installing the AccessoriesPreparing a Specimen Installing the Double-walled Buffer TrayInstalling and Removing the Blade Holder Now return the blade holder to the cutting position Inserting the Blade Adjusting the Clearance AngleWorking with the Instrument Operating the VibroCheck Using the VibroCheck with the VT1200Normal operating status is now restored Using the VibroCheck with the VT1200S Operating the VibroCheck Harm the instrument Malfunctions Meanings and TroubleshootingError Messages and Troubleshooting Error/INF No Troubleshooting RemarkReached C2 have different Software updatedInF Panel and keyboardBlade Dirty. CleanDown during installa VibroCheck VibroCheck was Collides withInstrument is Plug connections are Blocked when RUN is activeNot accepted Negative values Values of more than 1000μm Replacing the Main Fuse Clean using an alcohol-based solution or acetone Cleaning and MaintenanceCleaning the Instrument Display Buffer traySpecimen plate BladeBuffer Tray Extension Accessories for Standard SpecimensLid for covering the buffer tray Specimen PlateVibroCheck Magnifier, Complete Blades Microscope, CompleteInstalling the Magnifier Support or Microscope Support 240 V, 50/60 Hz Fiber-optic Illumination, Cold Light Source230-240 V, 50/60 Hz Voltage variant 230V/50/60Hz Leica VT1200/VT1200S Microtome with vibrating blade EC Declaration of ConformityWarranty Warranty and ServiceOperating Manual V1.1 05/2007