Leica Leica VT1200S manual Working with the Instrument, Description of the Typical Application

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5.Working with the Instrument

5.1Description of the Typical Application

The Leica VT1200 / VT1200S is a microtome with a vibrating blade and is predominately used for sectioning fixed and unfixed specimens during neu- rological research.

•To simplify the process of inserting the spec- imen, you can quickly move the specimen receptacle to the lowest position by pressing the DOWN button.

•To prepare high-quality sections, especially for unfixed tissue, Leica suggests using the optional VibroCheck measuring instrument to determine the height amplitude of the blade after each blade change, and then to minimize it using the setting screw on the blade holder.

To perform the aforementioned process, in- stall the VibroCheck instrument (see p. 36 for the VT1200 or p. 38 for the VT1200S), install the blade and adjust the clearance angle to the desired position. Then take the measure- ment and make the appropriate adjustments to the placement of the blade holder.

Remove the VibroCheck according to the in- structions, and turn the blade 90° toward the top to ensure that the ice bath and buffer tray can be installed safely.

•Insert the buffer tray into the ice bath and cover it with the lid. Fill the ice bath with crushed ice.

•Remove the cover and fill the buffer tray with pre-cooled physiological buffer.

•Push the ice bath and buffer tray onto the dovetail guide and clamp them down.

•Use cyanoacrylate adhesive to adhere the specimen to the specimen plate and use a manipulator to place it in the buffer tray.

Insert the hose for gassing the buffer into the hose clamp.

•Use the UP button to lift the specimen to the sectioning level of the blade quickly. Fine- adjusting the blade-to-specimen proximity can be performed using the desired step size 1, 10 or 100μm.

•Advance the blade toward the specimen us- ing the "Blade forward" key.

•After sectioning the specimen, use the setting dial to feed to the desired section thickness using the selected step size (1, 10 or 100μm). This raises the specimen receptacle to the desired value.

•Start the sectioning process by pressing the RUN/STOP key. The process can be stopped again by pressing this key after the cut has been completed. Use the "Blade back" key to move the blade in front of the specimen. Select the section thickness for the next cut and start the sectioning process again.

•After completing the sectioning process, remove the blade, dispose of the specimen receptacle by pushing the DOWN key into its lowest position, and unclamp, empty and clean out the ice bath and buffer tray.

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Operating Manual V1.1 – 05/2007

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Contents Leica VT1200 Leica VT1200S Page Nussloch Germany Contents Intended Use/Improper Use Symbols in the Text and their MeaningsImportant Notes Qualification of PersonnelSafety General Safety NotesTransport, Unpacking and Setting up Proper handlingInstrument Characteristics Technical Data for the VT1200Electrical data Dimensions Instrument CharacteristicsTechnical Data for the VT1200S Ambient conditionsGeneral Overview of the VT1200/VT1200S MicroscopeAttachments on dovetail receptacle Blades for the blade holderVT1200 Control Panel LED for feed rate1 VT1200S Control Panel Window edge is set Moves the specimen Upwards as long asInstallation Standard Scope of Delivery for the VT1200Standard Scope of Delivery for the VT1200S 048191200S001 Packing and Setting up the Instrument Packing the instrumentBefore Commissioning the Instrument Working with the Instrument Description of the Typical ApplicationControl Elements on the VT1200 Control Panel Moving the Specimen Receptacle VerticallyBlade Method Blade Forward backSelecting the Sectioning Parameters Starts the sectioning processBlade feed rate Speed LED illumination is switched onControl Element on the Control Panel of the VT1200S Selected amplitude Ampl Saved section thicknessToggling from Auto Toggling from MAN to An audible warning signal soundsWhen these keys are pressed ModeAutomatic mode max μm Adjusting the amplitude fromKey/setting dial Mm in 0.05 mm increments Not possibleWorking with the Instrument Blade forward and Blade Window edge key until the LED Key lights upWard key. Press the 1st cutting Ward key and press 2nd cuttingΜm display is then reset to ProcessOr 100μm by max μm using rotary knob Using predefined step sizes 1Automatic section thickness Stroke Single can be selected MAN with rotary knob 1 forStroke type CUT only single With rotary knob 1. If continuous5 mm/s in 0.5 mm/s increments Installing the Accessories Installing the Ice Bath and Buffer TrayInstalling the Double-walled Buffer Tray Preparing a SpecimenInstalling and Removing the Blade Holder Inserting the Blade Adjusting the Clearance Angle Now return the blade holder to the cutting positionWorking with the Instrument Using the VibroCheck with the VT1200 Operating the VibroCheckNormal operating status is now restored Using the VibroCheck with the VT1200S Operating the VibroCheck Error/INF No Troubleshooting Remark Malfunctions Meanings and TroubleshootingError Messages and Troubleshooting Harm the instrumentReached Panel and keyboard Software updatedInF C2 have differentVibroCheck VibroCheck was Collides with Dirty. CleanDown during installa BladeBlocked when RUN is active Not accepted Negative values Values of more than 1000μmInstrument is Plug connections are Replacing the Main Fuse Cleaning and Maintenance Cleaning the InstrumentClean using an alcohol-based solution or acetone Blade Buffer traySpecimen plate DisplayExtension Accessories for Standard Specimens Buffer TraySpecimen Plate Lid for covering the buffer trayVibroCheck Blades Microscope, Complete Magnifier, CompleteInstalling the Magnifier Support or Microscope Support Fiber-optic Illumination, Cold Light Source 230-240 V, 50/60 Hz240 V, 50/60 Hz Voltage variant 230V/50/60Hz EC Declaration of Conformity Leica VT1200/VT1200S Microtome with vibrating bladeWarranty and Service WarrantyOperating Manual V1.1 05/2007
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