Leica Mastering the VT1200S Microscope: Essential Working Instructions

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5.Working with the Instrument

Key/setting dial	semiautomatic sectioning mode	automatic sectioning mode

	The blade feed rate can be adjusted	ditto
1	from 0.01 – 1.5 mm/s:
	from 0.01 – 1.5 mm/s:
	0.01 – 0.1 in 0.01 mmm/s increments,
	0.10 – 0.5 in 0.02 mmm/s increments,
	0.50 – 1.5 in 0.10 mmm/s increments.

1	Adjusting the amplitude from	ditto
1	0 – 3 mm in 0.05 mm increments
	0 – 3 mm in 0.05 mm increments

1	Not possible.	Setting the section thickness for
		automatic mode – max. 1000μm.

Display for the current specimen ditto holder position (lowest position =

0μm, uppermost position = 20,000μm.) You can reset the display to zero at any point using the CLEAR/∑μm key. The section thicknesses are then added in the ∑μm display.

24	Operating Manual V1.1 – 05/2007

Image 24

Contents Leica VT1200 Leica VT1200S Page Nussloch Germany Contents Symbols in the Text and their Meanings Important NotesIntended Use/Improper Use Qualification of PersonnelSafety General Safety NotesTransport, Unpacking and Setting up Proper handlingInstrument Characteristics Technical Data for the VT1200Electrical data Instrument Characteristics Technical Data for the VT1200SDimensions Ambient conditionsGeneral Overview of the VT1200/VT1200S MicroscopeAttachments on dovetail receptacle Blades for the blade holderVT1200 Control Panel LED for feed rate1 VT1200S Control Panel Window edge is set Moves the specimen Upwards as long asInstallation Standard Scope of Delivery for the VT1200Standard Scope of Delivery for the VT1200S 048191200S001 Packing and Setting up the Instrument Packing the instrumentBefore Commissioning the Instrument Working with the Instrument Description of the Typical ApplicationControl Elements on the VT1200 Control Panel Moving the Specimen Receptacle VerticallyBlade Method Blade Forward back Selecting the Sectioning Parameters Starts the sectioning processLED illumination is switched on Control Element on the Control Panel of the VT1200SBlade feed rate Speed Selected amplitude Ampl Saved section thicknessToggling from MAN to An audible warning signal sounds When these keys are pressedToggling from Auto ModeAdjusting the amplitude from Key/setting dialAutomatic mode max μm Mm in 0.05 mm increments Not possibleWorking with the Instrument Blade forward and Blade Key lights up Ward key. Press the 1st cuttingWindow edge key until the LED Ward key and press 2nd cuttingΜm display is then reset to ProcessOr 100μm by max μm using rotary knob Using predefined step sizes 1Automatic section thickness MAN with rotary knob 1 for Stroke type CUT only singleStroke Single can be selected With rotary knob 1. If continuous5 mm/s in 0.5 mm/s increments Installing the Accessories Installing the Ice Bath and Buffer TrayInstalling the Double-walled Buffer Tray Preparing a SpecimenInstalling and Removing the Blade Holder Inserting the Blade Adjusting the Clearance Angle Now return the blade holder to the cutting positionWorking with the Instrument Using the VibroCheck with the VT1200 Operating the VibroCheckNormal operating status is now restored Using the VibroCheck with the VT1200S Operating the VibroCheck Malfunctions Meanings and Troubleshooting Error Messages and TroubleshootingError/INF No Troubleshooting Remark Harm the instrumentReached Software updated InFPanel and keyboard C2 have differentDirty. Clean Down during installaVibroCheck VibroCheck was Collides with BladeBlocked when RUN is active Not accepted Negative values Values of more than 1000μmInstrument is Plug connections are Replacing the Main Fuse Cleaning and Maintenance Cleaning the InstrumentClean using an alcohol-based solution or acetone Buffer tray Specimen plateBlade DisplayExtension Accessories for Standard Specimens Buffer TraySpecimen Plate Lid for covering the buffer trayVibroCheck Blades Microscope, Complete Magnifier, CompleteInstalling the Magnifier Support or Microscope Support Fiber-optic Illumination, Cold Light Source 230-240 V, 50/60 Hz240 V, 50/60 Hz Voltage variant 230V/50/60Hz EC Declaration of Conformity Leica VT1200/VT1200S Microtome with vibrating bladeWarranty and Service WarrantyOperating Manual V1.1 05/2007