Leica Leica VT1200S manual Blade Method, Blade Forward back

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5.Working with the Instrument

5.2.2 Fine-adjusting the Blade-to-Specimen proximity and Feeding to the Section Thickness

The rotary knob 2 for moving the specimen receptacle vertically can be used both for fine-adjusting the blade-to-specimen proximity and for adjusting the desired section thicknesses. The step sizes 1, 10 or 100μm can be se- lected by pressing the STEP SIZE key.

Turning the setting dial clockwise carries out the desired feed; turning the setting dial counterclockwise lowers the specimen (minus sign). The value appears in the lower display when "μm" is selected. After each sectioning process has been completed, the μm display is set to zero.

Save a commonly used section thickness by adjusting the setting dial for the section thickness and pressing the MEMO key for 3 seconds. An audi- ble signal confirms that the value has been accepted.

Feed to the saved value by quickly pressing the MEMO key. Pressing the MEMO multiple times feeds to the value multiple times.

Neither negative values nor a section thickness of over 1000μm are permitted. If an attempt is made to save impermissible values, an audible warning signal sounds three times and the last (permis- sible) value is retained.

5.2.3 Blade Method

The "Blade forward" and "Blade back" keys have to be held down until the desired position is reached. The blade feed rate is 2.5 mm/s. When each end point has been reached, the corresponding LED lights up in the key.

Blade

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20	Operating Manual V1.1 – 05/2007

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Contents Leica VT1200 Leica VT1200S Page Nussloch Germany Contents Symbols in the Text and their Meanings Important NotesIntended Use/Improper Use Qualification of PersonnelSafety General Safety NotesTransport, Unpacking and Setting up Proper handlingElectrical data Instrument CharacteristicsTechnical Data for the VT1200 Instrument Characteristics Technical Data for the VT1200SDimensions Ambient conditionsGeneral Overview of the VT1200/VT1200S MicroscopeAttachments on dovetail receptacle Blades for the blade holderVT1200 Control Panel LED for feed rate1 VT1200S Control Panel Window edge is set Moves the specimen Upwards as long asInstallation Standard Scope of Delivery for the VT120091200S001 Standard Scope of Delivery for the VT1200S0481 Packing and Setting up the Instrument Packing the instrumentBefore Commissioning the Instrument Working with the Instrument Description of the Typical ApplicationControl Elements on the VT1200 Control Panel Moving the Specimen Receptacle VerticallyBlade Method Blade Forward backSelecting the Sectioning Parameters Starts the sectioning processLED illumination is switched on Control Element on the Control Panel of the VT1200SBlade feed rate Speed Selected amplitude Ampl Saved section thicknessToggling from MAN to An audible warning signal sounds When these keys are pressedToggling from Auto ModeAdjusting the amplitude from Key/setting dialAutomatic mode max μm Mm in 0.05 mm increments Not possibleWorking with the Instrument Blade forward and Blade Key lights up Ward key. Press the 1st cuttingWindow edge key until the LED Ward key and press 2nd cuttingΜm display is then reset to ProcessAutomatic section thickness Or 100μm by max μm using rotary knobUsing predefined step sizes 1 MAN with rotary knob 1 for Stroke type CUT only singleStroke Single can be selected With rotary knob 1. If continuous5 mm/s in 0.5 mm/s increments Installing the Accessories Installing the Ice Bath and Buffer TrayInstalling the Double-walled Buffer Tray Preparing a SpecimenInstalling and Removing the Blade Holder Inserting the Blade Adjusting the Clearance Angle Now return the blade holder to the cutting positionWorking with the Instrument Using the VibroCheck with the VT1200 Operating the VibroCheckNormal operating status is now restored Using the VibroCheck with the VT1200S Operating the VibroCheck Malfunctions Meanings and Troubleshooting Error Messages and TroubleshootingError/INF No Troubleshooting Remark Harm the instrumentReached Software updated InFPanel and keyboard C2 have differentDirty. Clean Down during installaVibroCheck VibroCheck was Collides with BladeInstrument is Plug connections are Blocked when RUN is activeNot accepted Negative values Values of more than 1000μm Replacing the Main Fuse Clean using an alcohol-based solution or acetone Cleaning and MaintenanceCleaning the Instrument Buffer tray Specimen plateBlade DisplayExtension Accessories for Standard Specimens Buffer TraySpecimen Plate Lid for covering the buffer trayVibroCheck Blades Microscope, Complete Magnifier, CompleteInstalling the Magnifier Support or Microscope Support 240 V, 50/60 Hz Fiber-optic Illumination, Cold Light Source230-240 V, 50/60 Hz Voltage variant 230V/50/60Hz EC Declaration of Conformity Leica VT1200/VT1200S Microtome with vibrating bladeWarranty and Service WarrantyOperating Manual V1.1 05/2007