SSI America 90-2581 REV B manual Self-FlushSolution, Inlet Tubing and Filters

2.6.2 Self-Flush Solution

Self-flush heads require 250-500 mL of flushing solution. See

section 1.1.4 for self-flush solution recommendations. A pH indicator that will indicate the concentration of salts in the solution is recommended as a reminder to change the solution. This flush solution should be replaced with a fresh solution weekly to avoid frequent pump maintenance.

2.6.3 Inlet Tubing and Filters

The table below shows the inlet tubing and filter used in the Binary Solvent Delivery Module. All inlet lines are supplied in a 30" (76 cm) length and are made of a fluoropolymer material.

2.6.4 Priming the Pump and the Flushing Lines

Connect a syringe to the outlet tubing. Run the pump at a flowrate of 3 to 5 ml/min. Prime the pump by pulling mobile phase and any air bubbles through the system and into the syringe (a minimum of 20 ml).

To prime the flush lines for a self-flush head, simply place the inlet line in the flush solution and connect a syringe to the outlet line and apply suction until the line is filled with flush solution. Place the outlet line in the flush solution. Secure both flush lines in the flush solution container so they stay immersed during pump operation.

2.6.6 Long Term Pressure Calibration Accuracy

This note applies if your pump is equipped with an electronic pressure transducer. The transducer has been zeroed and calibrated at the factory. Over the life of the pump, some drift may occur. For example, it is typical for the zero to drift < 10 p.s.i. after about 1 year of operation (i.e., with no back pressure on the pump a reading of 1-9 p.s.i. may be displayed). A similar drift may also occur at higher pressures, and are typically less than 1% (e.g. <50 p.s.i. at 6,000 p.s.i. back pressure).

If pressure calibration and/or drift is a concern, consult the factory. The pump can be shipped back to SSI for recalibration. Alternatively, written calibration and zero-reset procedures are available. Consult the factory to receive these instructions.

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