Gilson 215 manual Pipetting Techniques

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Pipetting Techniques

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By following these techniques, you’ll be able to maximize accuracy and precision, and minimize carryover, with your Quad-Z 215 Liquid Handler.

Accuracy and precision of pipetting are dependent on the specifications of the pump you’re using and the geometry of the probe tip.

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Contents Quad-Z 215 Liquid Handler Users Guide Page Table of Contents Operation MaintenanceAppendix a Replacement Parts and Accessories TroubleshootingAppendix C Gsioc Commands Appendix B RacksPage Declaration of Conformity Page Introduction1 Description Unpacking Standard Equipment Customer Service Technical Specifications Introduction Introduction Installation2 Installation Locator Plate InstallationArmlock Removal Arm Installation Installation Installing the Z-Arm Cable Support Rod Installing the Probe Guide Inserts Probe InstallationInstalling the Probes Plumbing Connections Transfer Tubing InstallationTo install the level sensing cable Installing the Level Sensing CablesPlumbing Connections Installing the rinse station Code 200-Series Code 500-Series Racks Rack SetupCode 200-Series and Code 20-Series or Code 30-Series Racks Installation Only Code 20-Series or Code 30-Series Racks Part number Description Final Z-Arm AdjustmentFinal Z-ArmAdjustment Rear Panel Electrical ConnectionsContact inputs Installation Input/Output PortsContact outputs Items you’ll needMaking connections Connecting an RS-232 cable Installation RS-232 PortGsioc cable Gsioc PortUnit ID Installation Unit ID and Baud Rate/Mode SelectionBaud rate/mode Installation Fuses Power Cord Connection Operation Front Panel Start Up Running Programs Configuring the Liquid Handler About Syringe Options Operation Pump TypeRinse Site Operation Liquid Detector Adjust XY Model 841 X-coordinate 475.3 mm Y-coordinate 3.8 mm Height Operation Home Phase Bearing Life Safety ContactEmergency Contact Operation Sound and DisplayTesting the Liquid Handler’s Contacts Maintenance4 Helpful Hints MaintenanceCleaning the Liquid Handler CleaningCleaning the Fluid Path Cleaning methods Maintenance Maintenance Lubricating ProceduresHorizontal Pitch and Vertical Rods What you needMm spacing Installing same type of probe Replacing PartsReplacing Tubing Replacing a ProbeInstalling different type of probe Removing the level sensing cable Maintenance Replacing a Level Sensing CableInstalling the new level sensing cable Maintenance Replacing a Fuse Checking Position Alignment Transporting the Liquid Handler Page Troubleshooting5 Troubleshooting Error MessagesPosition sensor inactive Probes no longer finding tube center MechanicalInput functions not operating ElectricalOutput functions not operating Unit blows fuses Unit not operationalLiquid level detector not detecting liquid level Liquid Level DetectorLiquid level detector falsely detecting liquid level Non-warranty repair Warranty repairRepair and Return Policies Before calling usRebuilt exchange Return procedurePage Probes Replacement Parts and AccessoriesProbe Guide Inserts Control SoftwareTransfer Tubing and Waste Bottle AppendixRinse Station Rack AccessoriesMiscellaneous Cables and I/O AccessoriesRacks Code 21 rack Code 20 rackCode 23 rack Code 24 rackCode 29 rack Code 28 rackCode 30 rack Code 31 rackCode 33 rack Code 32 rackCode 200 rack Code 201H rack Code 201 rackCode 202 rack Code 203 rackCode 205 rack Code 204 rackCode 205H rack Code 207 rack Code 206 rackCode 208 rack Code 209 rackCode 211 rack Code 210 rackCode 212 rack Code 213 rackCode 216 rack Code 214 rackCode 217 rack Code 218 rackCode 228 rack Code 222 rackCode 505 rack Code 505H rackCode 242 Peltier rack Appendix Peltier RacksCode 542 Peltier rack Code 853 Peltier rack Code 852 Peltier rackCode 854 Peltier rack Gsioc Commands Gsioc Command List Gsioc CommandsCommand Type Description @aa=xxxx Bf,d Cv=ss..ss Jccccp ExyzJcttt Ksxxx Oabcd Smm Ta,b,c,d Wcc..cc Vcs..sXax/y Xbx/y Xcx/y Xdx/y Yyyyy Zsp ZspB Pipetting Techniques Minimizing Cross-Contamination Minimizing Dilution Effect Appendix