D
The following example describes an instance when the above 8-step procedure is required. An operator needs to dispense 50 µL of reagent into each of 20 test tubes. The tube-to-tube consistency of reagent volume is critical. However, in order to save time, the operator wishes to make a single 1 mL aspiration from the reagent reservoir and then make multiple dispenses. If the 8-step procedure was not used, the last aliquot of the 20 dispenses would be more diluted than the others. If the procedure was used, the requirements of the routine and the operator’s desire to save time could both be met. (Recommended volumes in this example are 15 µL air gaps and 100 µL excess volume in Step 4.)
