Leica RM2245 instruction manual Sectioning mode, Trimming mode, How to activate the Step function

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5. Operation

Sectioning mode

Button functions in STEP

mode

Multiple Single

step step

backward backward

Multiple Single

step step

forward forward

Trimming mode

Backwards coarse feed

40

Forward coarse feed

41

In sectioning mode the user can select between STEP function (step- by-step specimen feed) and continuous specimen feed.

When continuous feed is selected, the coarse feed buttons have the same func- tions as in trimming mode. The STEP function is useful for careful step-by-step approximation of the specimen towards the blade.

How to activate the STEP function:

•Switch the instrument on while holding the button on the control panel. (Likewise, to deactivate switch the instrument on while pressing the button

.) While the instrument is initializing, hold the button until the software version number is no longer displayed (ensure that version number 2.1 was displayed).

This feature is only available in sectioning mode in version 2.1 or higher. If you have an earlier software version, please contact Leica Service.

•PressTRIM/SECT buttonandselectsectioningmode(LEDSECTlit).

•When pressing a slow-speed coarse feed button (with an arrow) in STEP mode, the specimen moves towards or away from the specimen by the value indicated on the display (single step).

•By short activation of a double-arrow coarse feed button, a single step is also effected in the appropriate direction.

•Longer activation of the double-arrow coarse feed button effects a repeated feed motion for as long as the button is pressed.

In the trimming mode, the coarse feed buttons operate a continuous movement as long as the button is held down. The double-arrow button for rapid coarse feed backward movements has a lock-in function.

•To start the rapid backwards movement (away from the blade) press the

button .

After the button is pressed, the object head is moved to the rear end position.

•To stop the movement, press any of the four coarse feed buttons.

•The yellow LED (40) in the button flashes while the object head is in motion, and remains lit continuously when the rear end position is reached.

•Press the button to start the slow backwards movement. The travel continues as long as the button is held depressed.

•Press the appropriate button to start a rapid or slow forward movement. The travel continues as long as the button is held depressed.

•During the forwards movement, the yellow LED (41) in the button flashes. When the front end position is reached, an acoustic signal is heard and the LED stops flashing and remains lit.

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Instruction manual V 1.4 – 02/2007

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Contents Leica RM2245 Page Leica Microsystems Nussloch GmbH Table of Contents Table of Contents Symbols in the text and their meanings Important notesQualification of personnel Intended use of instrumentSafety Safety notesPersonal safety precautions Safety Integrated safety devices Safety Instrument components and specifications Overview instrument componentsLeica RM2245 Instrument specifications Rear side of instrumentTechnical data General Dimensions and weight Basic instrumentControl panel Microtome Startup Standard deliveryInstallation site requirements Setting up the instrumentAssembling the handwheel Electrical connectionsChecking the voltage 23 24 Switching on the instrument Operation Operating elements and their functionsInstrument control panel Control panel Display and control elements Sectioning mode Trimming modeHow to activate the Step function Specimen retraction Mum of 100 μm using the buttons on the control panel Display 4 lights upStep function is disabled in the remaining feed range Indication of remaining horizontal feedObject head Directional fixture for specimen clamps To release the clamp, turn the eccentric lever 29 forwardsImportant! Never turn the screw more than 1/2 Turn at a time Inserting the knife holder Inserting the universal cassette clamp Adjusting the clearance angle Example Enlarged detail showing a clearance angle settingClamping the specimen Clamping the knife / disposable bladeCutting into the specimen trimming Sectioning Changing the specimen or interrupting sectioning Finishing daily routineOptional accessories Assembly for fixture for specimen clampsRigid fixture for specimen clamps Directional fixture for specimen clampsFine-directional fixture for specimen clamps Quick clamping system Specimen clamps and holders Standard specimen clampVee insert Foil clamp type Optional accessories Universal cassette clamp Universal cassette clamp, ice-cooledSuper mega-cassette clamp Holder for round specimens Optional accessories Knife holder E/E-TC To clamp the blade, rotate clamping lever 10 back upwardsOptional accessories Use Knife holder N/NZ Optional accessories Section waste tray BacklightingTray Freezer packInstrument Universal microscope carrier Assembling the universal microscope carrierAttach baseplate Attach horizontal arm Magnifying lens Plug the power plug into the power socket Cold light source Fiber-optic light guideOptional accessories Ordering informationCold light sources Leica CLSFiber-optic light guide Customized solutions Error reportsInstrument malfunctions Malfunctions, possible causes and troubleshooting Problem Possible cause Corrective actionPossible faults Cleaning and maintenance Cleaning the instrumentClean using an alcohol-based solution or acetone Cleaning and maintenance Maintenance Replacing fusesMaintenance instructions Lubricating the instrument Warranty and service WarrantyEC Declaration of Conformity We herewith declare, in exclusive responsibility, thatDag Graupner Managing Director
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