Leica RM2245 instruction manual Inserting the knife holder

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5. Operation

5.2 Inserting the knife holder

55

 

53

 

 

50

52

 

Enlarged detail:

 

Scale for better

51

repositioning of the

 

knife holder for

 

varying specimen

 

heights.

54

Fig. 20

Setting up the knife holder base

Release the clamping lever (50) by rotating it counterclockwise.

Insert the knife holder base (51) using the notch (52) on the bottom into the T-piece (55) of the microtome base plate (53).

To secure the knife holder base, turn the clamping lever (50) clockwise.

The knife holder base (51) can be moved back and forth on the microtome base plate. This allows bringing the knife holder to optimal sectioning position in relation to the specimen.

There is a scale (54) on the right side of the microtome base plate. This enables faster and better positioning of the knife holder at the specimen if various combinations of standard specimens and specimen holders are used. The rear edge of the knife holder base (51) functions as the scale reference.

57

56 71

58

Fig. 21

Inserting the knife holder

Loosen the screw (58) using an Allen key size 4 (71) until the knife holder (57) can be moved.

Place the knife holder (57) with the under- side groove onto the T-piece (56) of the knife holder base (51).

To clamp, retighten the screw (58).

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Instruction manual V 1.4 – 02/2007

Image 30
Contents Leica RM2245 Page Leica Microsystems Nussloch GmbH Table of Contents Table of Contents Qualification of personnel Symbols in the text and their meaningsImportant notes Intended use of instrumentSafety Safety notesPersonal safety precautions Safety Integrated safety devices Safety Instrument components and specifications Overview instrument componentsLeica RM2245 Instrument specifications Rear side of instrumentTechnical data General Dimensions and weight Basic instrumentControl panel Microtome Startup Standard deliveryInstallation site requirements Setting up the instrumentAssembling the handwheel Electrical connectionsChecking the voltage 23 24 Switching on the instrument Operation Operating elements and their functionsInstrument control panel Control panel Display and control elements Sectioning mode Trimming modeHow to activate the Step function Specimen retraction Mum of 100 μm using the buttons on the control panel Display 4 lights upStep function is disabled in the remaining feed range Indication of remaining horizontal feedObject head Directional fixture for specimen clamps To release the clamp, turn the eccentric lever 29 forwardsImportant! Never turn the screw more than 1/2 Turn at a time Inserting the knife holder Inserting the universal cassette clamp Adjusting the clearance angle Example Enlarged detail showing a clearance angle settingClamping the specimen Clamping the knife / disposable bladeCutting into the specimen trimming Sectioning Changing the specimen or interrupting sectioning Finishing daily routineRigid fixture for specimen clamps Optional accessoriesAssembly for fixture for specimen clamps Directional fixture for specimen clampsFine-directional fixture for specimen clamps Quick clamping system Specimen clamps and holders Standard specimen clampVee insert Foil clamp type Optional accessories Universal cassette clamp Universal cassette clamp, ice-cooledSuper mega-cassette clamp Holder for round specimens Optional accessories Knife holder E/E-TC To clamp the blade, rotate clamping lever 10 back upwardsOptional accessories Use Knife holder N/NZ Optional accessories Section waste tray BacklightingTray Freezer packInstrument Universal microscope carrier Assembling the universal microscope carrierAttach baseplate Attach horizontal arm Magnifying lens Plug the power plug into the power socket Cold light source Fiber-optic light guideOptional accessories Ordering informationCold light sources Leica CLSFiber-optic light guide Customized solutions Error reportsInstrument malfunctions Malfunctions, possible causes and troubleshooting Problem Possible cause Corrective actionPossible faults Cleaning and maintenance Cleaning the instrumentClean using an alcohol-based solution or acetone Cleaning and maintenance Maintenance Replacing fusesMaintenance instructions Lubricating the instrument Warranty and service WarrantyEC Declaration of Conformity We herewith declare, in exclusive responsibility, thatDag Graupner Managing Director