Leica RM2245 instruction manual Directional fixture for specimen clamps

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5. Operation

Directional fixture for specimen clamps

In the quick clamping device of the directional specimen holder fixture, all specimen clamps available as optional accessories can be used (implemented).

The object orientation allows for simple position correction of the specimen surface when the specimen is clamped into place.

30

32

32

31

29

Fig. 17

Display of the zero position

For better display of the zero position, the orien- tation has two red indicators (32).

When both indicators are visible and both set- screws are in zero position at the same time (notch point, white marking on "￿"), the speci- men is in zero position.

When the large standard specimen clamp (50 x 55 mm) is used, the speci- men orientation of 8° in north-south direction is no longer possible.

The usable angle is only about 4° in this case.

The directional specimen holder fixture may be exchanged for a non-directional fixture (optional accessory).

Orienting the specimen

Specimen blocks must not be oriented during the retraction phase!

If a block is oriented during retraction, the block will advance by the retraction value PLUS the selected section thick- ness before the next section.

This may cause damage to both specimen and knife!

Raise the object head to the upper end posi- tion and activate the handwheel lock.

To release the clamp, turn the eccentric lever (29) forwards.

Turn setscrew (30) to orient the specimen in north-south direction. Turn setscrew (31) to orient the specimen in east-west direction.

Each complete turn of the screw inclines the specimen by 2°. A total of 4 complete turns = 8° are possible in every direction. The accu- racy is approximately ± 0.5°.

For ease of estimation, there is a white marking on the handle and a notch point that is noticeable during turning.

To lock the current orientation, turn the ec- centric lever (29) backwards.

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Instruction manual V 1.4 – 02/2007

Image 28
Contents Leica RM2245 Page Leica Microsystems Nussloch GmbH Table of Contents Table of Contents Symbols in the text and their meanings Important notesQualification of personnel Intended use of instrumentSafety Safety notesPersonal safety precautions Safety Integrated safety devices Safety Overview instrument components Instrument components and specificationsLeica RM2245 Instrument specifications Rear side of instrumentDimensions and weight Basic instrument Technical data GeneralControl panel Microtome Startup Standard deliveryInstallation site requirements Setting up the instrumentElectrical connections Assembling the handwheelChecking the voltage 23 24 Switching on the instrument Operating elements and their functions OperationInstrument control panel Control panel Display and control elements Trimming mode Sectioning modeHow to activate the Step function Specimen retraction Mum of 100 μm using the buttons on the control panel Display 4 lights upIndication of remaining horizontal feed Step function is disabled in the remaining feed rangeObject head Directional fixture for specimen clamps To release the clamp, turn the eccentric lever 29 forwardsImportant! Never turn the screw more than 1/2 Turn at a time Inserting the knife holder Inserting the universal cassette clamp Adjusting the clearance angle Example Enlarged detail showing a clearance angle settingClamping the specimen Clamping the knife / disposable bladeCutting into the specimen trimming Sectioning Changing the specimen or interrupting sectioning Finishing daily routineOptional accessories Assembly for fixture for specimen clampsRigid fixture for specimen clamps Directional fixture for specimen clampsFine-directional fixture for specimen clamps Quick clamping system Standard specimen clamp Specimen clamps and holdersVee insert Foil clamp type Optional accessories Universal cassette clamp Universal cassette clamp, ice-cooledSuper mega-cassette clamp Holder for round specimens Optional accessories Knife holder E/E-TC To clamp the blade, rotate clamping lever 10 back upwardsOptional accessories Use Knife holder N/NZ Optional accessories Section waste tray BacklightingFreezer pack TrayInstrument Assembling the universal microscope carrier Universal microscope carrierAttach baseplate Attach horizontal arm Magnifying lens Plug the power plug into the power socket Cold light source Fiber-optic light guideOptional accessories Ordering informationLeica CLS Cold light sourcesFiber-optic light guide Error reports Customized solutionsInstrument malfunctions Malfunctions, possible causes and troubleshooting Problem Possible cause Corrective actionPossible faults Cleaning the instrument Cleaning and maintenanceClean using an alcohol-based solution or acetone Cleaning and maintenance Maintenance Replacing fusesMaintenance instructions Lubricating the instrument Warranty and service WarrantyWe herewith declare, in exclusive responsibility, that EC Declaration of ConformityDag Graupner Managing Director