Leica RM2245 instruction manual Cutting into the specimen trimming Sectioning

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5.Operation

5.7 Cutting into the specimen (trimming)

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5.8 Sectioning

Use the TRIM/SECT key to select the trim mode.

Set the desired trim section thickness.

Release the handwheel lock and the brake.

In TRIM mode, use the coarse feed buttons to move the sample against the knife/blade.

Trim the sample by turning the handwheel or

Press the ROCK button (LED (41) in button is lit) to select ROCK mode. Trim the specimen by rocking the handwheel back and forth.

Terminate trimming when the desired sectioning surface and depth have been reached.

Always turn the handwheel evenly in clockwise direction; otherwise, the brake will not work properly. The rotation speed of the handwheel must be adapted to suit the hardness of the specimen. For harder specimens, use a slower speed.

Always use a different area of the cutting edge for trimming and sectioning. To do so, laterally displace the blade or knife in the knife holder.

When using the knife holder E with lateral movement, it is sufficient to move the knife holder sideways.

• Use the TRIM/SECT key to select the sectioning mode.

• Adjust the appropriate sectioning thickness or verify the selected value.

• Select conventional sectioning or ROCK mode.

To section the specimen, turn the handwheel clockwise smoothly or rock it back and forth if in ROCK mode.

Pick up the sections and mount them on microscope slides.

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Instruction manual V 1.4 – 02/2007

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Contents Leica RM2245 Page Leica Microsystems Nussloch GmbH Table of Contents Table of Contents Qualification of personnel Symbols in the text and their meaningsImportant notes Intended use of instrumentSafety Safety notesPersonal safety precautions Safety Integrated safety devices Safety Overview instrument components Instrument components and specificationsLeica RM2245 Instrument specifications Rear side of instrumentDimensions and weight Basic instrument Technical data GeneralControl panel Microtome Startup Standard deliveryInstallation site requirements Setting up the instrumentElectrical connections Assembling the handwheelChecking the voltage 23 24 Switching on the instrument Operating elements and their functions OperationInstrument control panel Control panel Display and control elements Trimming mode Sectioning modeHow to activate the Step function Specimen retraction Mum of 100 μm using the buttons on the control panel Display 4 lights upIndication of remaining horizontal feed Step function is disabled in the remaining feed rangeObject head Directional fixture for specimen clamps To release the clamp, turn the eccentric lever 29 forwardsImportant! Never turn the screw more than 1/2 Turn at a time Inserting the knife holder Inserting the universal cassette clamp Adjusting the clearance angle Example Enlarged detail showing a clearance angle settingClamping the specimen Clamping the knife / disposable bladeCutting into the specimen trimming Sectioning Changing the specimen or interrupting sectioning Finishing daily routineRigid fixture for specimen clamps Optional accessoriesAssembly for fixture for specimen clamps Directional fixture for specimen clampsFine-directional fixture for specimen clamps Quick clamping system Standard specimen clamp Specimen clamps and holdersVee insert Foil clamp type Optional accessories Universal cassette clamp Universal cassette clamp, ice-cooledSuper mega-cassette clamp Holder for round specimens Optional accessories Knife holder E/E-TC To clamp the blade, rotate clamping lever 10 back upwardsOptional accessories Use Knife holder N/NZ Optional accessories Section waste tray BacklightingFreezer pack TrayInstrument Assembling the universal microscope carrier Universal microscope carrierAttach baseplate Attach horizontal arm Magnifying lens Plug the power plug into the power socket Cold light source Fiber-optic light guideOptional accessories Ordering informationLeica CLS Cold light sourcesFiber-optic light guide Error reports Customized solutionsInstrument malfunctions Malfunctions, possible causes and troubleshooting Problem Possible cause Corrective actionPossible faults Cleaning the instrument Cleaning and maintenanceClean using an alcohol-based solution or acetone Cleaning and maintenance Maintenance Replacing fusesMaintenance instructions Lubricating the instrument Warranty and service WarrantyWe herewith declare, in exclusive responsibility, that EC Declaration of ConformityDag Graupner Managing Director