Leica Optional Accessories for Enhanced Use of RM2245 Water Bath

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6.Optional accessories

Knife holder E with water bath for narrow-band and broad-band blades

11

9

10

 

Knife holder E with water bath is available for both narrow-band and broad-band blades.

The knife guard on knife holder E consists of a red foldaway handle (9). To cover the cutting edge, fold the knife guard handle (9) upwards as illustrated in Figure.

Fig. 42

The clamping levers on the knife holder are not interchangeable. The two clamping levers (10 and 11) must remain in the position shown at all times, as otherwise isolated malfunctions of the knife holder can occur.

Clamping lever for the blade (10) at the right, clamping lever for the lateral displacement (11) at the left.

Use

Floating thin paraffin sections (for example, for subsequent immunostaining procedures) on the surface of the water. Flat paraffin sections can be removed from the surface of the water using glass slides.

The vessel is filled with water up to the blade. After trimming, remove the section waste from the tray and create the sections to be prepared. The sections floating on the surface of the wa- ter can be removed using the glass slide.

Fig. 43

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Instruction manual V 1.4 – 02/2007

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Contents Leica RM2245 Page Leica Microsystems Nussloch GmbH Table of Contents Table of Contents Symbols in the text and their meanings Important notesQualification of personnel Intended use of instrumentSafety Safety notesPersonal safety precautions Safety Integrated safety devices Safety Instrument components and specifications Overview instrument componentsLeica RM2245 Instrument specifications Rear side of instrumentTechnical data General Dimensions and weight Basic instrumentControl panel Microtome Startup Standard deliveryInstallation site requirements Setting up the instrumentAssembling the handwheel Electrical connectionsChecking the voltage 23 24 Switching on the instrument Operation Operating elements and their functionsInstrument control panel Control panel Display and control elements Sectioning mode Trimming modeHow to activate the Step function Specimen retraction Mum of 100 μm using the buttons on the control panel Display 4 lights upStep function is disabled in the remaining feed range Indication of remaining horizontal feedObject head Directional fixture for specimen clamps To release the clamp, turn the eccentric lever 29 forwardsImportant! Never turn the screw more than 1/2 Turn at a time Inserting the knife holder Inserting the universal cassette clamp Adjusting the clearance angle Example Enlarged detail showing a clearance angle settingClamping the specimen Clamping the knife / disposable bladeCutting into the specimen trimming Sectioning Changing the specimen or interrupting sectioning Finishing daily routineOptional accessories Assembly for fixture for specimen clampsRigid fixture for specimen clamps Directional fixture for specimen clampsFine-directional fixture for specimen clamps Quick clamping system Specimen clamps and holders Standard specimen clampVee insert Foil clamp type Optional accessories Universal cassette clamp Universal cassette clamp, ice-cooledSuper mega-cassette clamp Holder for round specimens Optional accessories Knife holder E/E-TC To clamp the blade, rotate clamping lever 10 back upwardsOptional accessories Use Knife holder N/NZ Optional accessories Section waste tray BacklightingTray Freezer packInstrument Universal microscope carrier Assembling the universal microscope carrierAttach baseplate Attach horizontal arm Magnifying lens Plug the power plug into the power socket Cold light source Fiber-optic light guideOptional accessories Ordering informationCold light sources Leica CLSFiber-optic light guide Customized solutions Error reportsInstrument malfunctions Malfunctions, possible causes and troubleshooting Problem Possible cause Corrective actionPossible faults Cleaning and maintenance Cleaning the instrumentClean using an alcohol-based solution or acetone Cleaning and maintenance Maintenance Replacing fusesMaintenance instructions Lubricating the instrument Warranty and service WarrantyEC Declaration of Conformity We herewith declare, in exclusive responsibility, thatDag Graupner Managing Director