Leica RM2245 instruction manual Clamping the specimen, Clamping the knife / disposable blade

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5. Operation

5.5 Clamping the specimen

Always clamp the specimen block BEFORE clamping the knife. Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of knife or specimen, prior to changing the specimen block and during all work breaks!

Rotate the handwheel until the specimen clamp is in the uppermost position.

Activate the handwheel lock by allowing the handwheel handle to lock in place and then activate the brake.

Insert a specimen block into the specimen clamp.

A detailed description for inserting the specimen into various specimen clamps and specimen holders is provided in Chapter 6, "Optional accessories".

5.6 Clamping the knife / disposable blade

Be very careful when handling microtome knives or blades. The cutting edge is extremely sharp and can cause severe injury!

Carefully insert knife or disposable blade into the knife holder and clamp.

Make sure that the blade is clamped parallel to the upper edge of the pressure plate.

(For more information see Chapter 6.2.2, Fig. 36)

A detailed description for inserting the blade or the knife into the individual knife holders is provided in Chapter 6, "Optional acces- sories".

Leica RM2245

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Contents Leica RM2245 Page Leica Microsystems Nussloch GmbH Table of Contents Table of Contents Important notes Symbols in the text and their meaningsQualification of personnel Intended use of instrumentSafety notes SafetyPersonal safety precautions Safety Integrated safety devices Safety Instrument components and specifications Overview instrument componentsLeica RM2245 Rear side of instrument Instrument specificationsTechnical data General Dimensions and weight Basic instrumentControl panel Microtome Standard delivery StartupSetting up the instrument Installation site requirementsAssembling the handwheel Electrical connectionsChecking the voltage 23 24 Switching on the instrument Operation Operating elements and their functionsInstrument control panel Control panel Display and control elements Sectioning mode Trimming modeHow to activate the Step function Specimen retraction Display 4 lights up Mum of 100 μm using the buttons on the control panelStep function is disabled in the remaining feed range Indication of remaining horizontal feedObject head To release the clamp, turn the eccentric lever 29 forwards Directional fixture for specimen clampsImportant! Never turn the screw more than 1/2 Turn at a time Inserting the knife holder Inserting the universal cassette clamp Example Enlarged detail showing a clearance angle setting Adjusting the clearance angleClamping the knife / disposable blade Clamping the specimenCutting into the specimen trimming Sectioning Finishing daily routine Changing the specimen or interrupting sectioningAssembly for fixture for specimen clamps Optional accessoriesRigid fixture for specimen clamps Directional fixture for specimen clampsFine-directional fixture for specimen clamps Quick clamping system Specimen clamps and holders Standard specimen clampVee insert Foil clamp type Optional accessories Universal cassette clamp, ice-cooled Universal cassette clampSuper mega-cassette clamp Holder for round specimens Optional accessories To clamp the blade, rotate clamping lever 10 back upwards Knife holder E/E-TCOptional accessories Use Knife holder N/NZ Optional accessories Backlighting Section waste trayTray Freezer packInstrument Universal microscope carrier Assembling the universal microscope carrierAttach baseplate Attach horizontal arm Magnifying lens Cold light source Fiber-optic light guide Plug the power plug into the power socketOrdering information Optional accessoriesCold light sources Leica CLSFiber-optic light guide Customized solutions Error reportsInstrument malfunctions Problem Possible cause Corrective action Malfunctions, possible causes and troubleshootingPossible faults Cleaning and maintenance Cleaning the instrumentClean using an alcohol-based solution or acetone Cleaning and maintenance Replacing fuses MaintenanceMaintenance instructions Lubricating the instrument Warranty Warranty and serviceEC Declaration of Conformity We herewith declare, in exclusive responsibility, thatDag Graupner Managing Director