Leica
DM4000B, DM4000M
manual
Leica DM4000 B
Troubleshooting
Install
Correction for Vision Problems
Optional Accessories
Assembly
Imaging Procedure
Torque adjustment
Checking Phase Contrast Rings
Cleaning Glass Surfaces
Ambient temperature 15-35C
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Leica DM4000 B
Leica DM4000 M
Leica DM5000 B
Operating Manual
3
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Contents
Leica DM4000 B
Page
Leica DM4000 B
Copyrights
Copyrights
Contents
Imaging Procedure for Essential
Explanatory note
Text symbols and their meanings → p
Important Notes about this Manual
Important Notes about this Manual
Microscope
Safety Notes
Safety Notes
General Safety Notes
Overvoltage category Pollution degree See enclosed manual
Safety Notes Supply unit ebq
Ambient temperature 15-35C Relative humidity
Ambient temperature 15-35C
Overview of the Instrument
CTR5000
Control Panels
Computer Interface
Software Tools
13 12 11 10 9
Overview of the Instrument
Unpacking the Microscope
Unpacking the Microscope
Installation location
Transport
Remove all moving or loose parts
Assembly
This case, read Chapter Optional accessories → p
Assembling the Microscope
Assembling the stage Stage clamp Dovetail guide
Assembly Stage
Connect the condenser over the connection 8.1 with the stand
Assembly Condenser
Objectives
Assembly Tube and Eyepieces
Remove the lamp’s dust cover
107/2 Lamp Housing
Lamp housing
18.4
Close the lamp housing and retighten the fas- tening screws
200 hrs
Please regard the data sheets of the burners
Type
High-pressure mercury burner alternating current 100 hrs
Install the burner in mirror image fashion
Hg 50 burner
Xe 75 burner
Rear side of the ebq 100 supply unit Lamp connection
Connect the lamp housing to the power supply
Equipping the Incident Light filter turret
Polarizer and Analyzer
Assembly ICT/P transmitted light polarizer
Motorized polarizer
Transmitted light and incident light analyzer
DIC Prisms
Optional Accessories
Motorized analyzer
Identification Sheet for the correct position
Booster Lens / Excitation Manager
Assembly Ergomodule
Mirror Housing
Connection to the Power Supply
Connection to the CTR5000 Electronics Box
Functional Principle
Switching on the Microscope
Startup
Startup
Combination mode
Phase contrast Transmitted light
Switch through all transmitted light processes
Switch through fluorescence cubes in clockwise fashion
Function key Meaning
Display Leica DM4000 B/DM4000 M
Function Keys
Light Intensity
Diaphragms Contrast Method
Magnification
Defined Function Keys on the left side of the stand
Abbreations are listed on p.32f
Köhler Illumination
Insert the specimen in the stage’s specimen holder
Set the light intensity using the INT keys
Köhler Illumination
Checking Phase Contrast Rings
Phlr
Light ring centering Clamping screw
Cube. See → p Turn the reflector into the light path
Never look directly into the light path
Centering the 12 V 100 W Halogen Lamp
Centering the Hg 50 W mercury lamp
Focus the direct filament image with the col- lector
Turn off the microscope before exchanging
Reflector cube
Focus the direct image with the collector
Centering the Hg 100 W and Xe 75 W
Mercury lamps
Startup
Startup
Torque adjustment
Operation
Operation
Operation Rotating the Stage
Focusing
Operation Tubes
Adjusting the Viewing Distance
Adjusting the Viewing Angle
Adjusting the Eyepiece Extension to the Arm Length
Eyepieces with Inlaid Reticle
Correction for Vision Problems
Operation Beam Splitting in Photo Tubes
Eyepieces
Operation Objectives
Follow safety instructions for immersion oil
Stand
Operation For lockable immersion objectives
For objectives with corrective mounts
Magnification Changer
Operation Light Sources
Phase Contrast
Imaging Procedure
Dark Field TL
Imaging Procedure for Leica DM4000 B/DM5000 B
Polarization TL
Mechanical procedure
Motorized procedure
Combined procedure
Differential Interference Contrast TL
Cube or Cube
Fluorescence
Dark Field
Imaging Procedure for Leica DM4000 M
Polarization
Automatic procedure
Imaging Procedure for Leica DM4000 M Interference Contrast
Trouble Shooting
Problem Cause/Remedy Stand
Illumination
→ p
Trouble Shooting Problem Cause/Remedy Bright Field
Display
Problem Cause/Remedy Polarization
Care of the Microscope
Handling Acids and Bases
Cleaning Glass Surfaces
Cleaning Objectives
Removing Immersion Oil
Wear and Spare Parts
Essential Wear and Spare Parts
Abbreviations and Pictograms
Abbreviations and Pictograms
Index
Index
EU Declaration of Conformity
EU Declaration of Conformity
Related manuals
Manual
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