Leica DM4000B, DM4000M Light Intensity, Diaphragms Contrast Method, Magnification, Function Keys

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6. Startup

The display shows the current microscope set- tings. The display depends on the microscope’s configuration. In the first column, corresponding pictograms indicate the type of information: con- trast method, magnification, light intensity, dia- phragms, light splitting for photo tubes.

Please see the abbreviation index for a list of ab- breviations and pictograms used → p. 67.

Light Intensity

The actual brightness setting is graphically de- picted by a beam. Additionally, the light intensity is indicated in 20 (coarse adjustment) or in 255 (fine adjustment) increments → p. 52.

Diaphragms

Contrast Method

In the first row, you find an indication of the ac- tive light axis (transmitted light or incident light) of the current contrast method and the current filter cube.

The shutter status is displayed for the transmitted light or incident light shutter:

Transmitted light shutter open

↑Transmitted light shutter closed

↑

Incident light shutter open

↓Incident light shutter closed

Magnification

+

The current objective magnification, sometimes followed by the re-magnification of the magnifi- cation changer, appears along with the total magnification:

Σ= Objective x Re-magnification x Eyepiece

6.4 The Function Keys

The values for the field diaphragm (FD) and the aperture diaphragm (AP) are indicated numeri- cally. The field diaphragm may be either round or rectangular. Accordingly, the FD designation is set in parentheses or in brackets: (FD) or [FD].

Note:

When using a digital camera, rectangular field diaphragms are recommended.

Beam splitting

If a motorized tube is used, the light splitting between ocular (Eye) and photo output (Docu) is indicated in %.

Note:

The display may flash after the initialization phase or even during microscopy session. This always occurs when the contrast method selected can not be performed with the actual microscopic settings. For example, an objective may be swiveled in that is not suited to the contrast method chosen.

Then check your settings.

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Explanatory note Text symbols and their meanings → pImportant Notes about this Manual Important Notes about this ManualMicroscope Safety NotesSafety Notes General Safety NotesOvervoltage category Pollution degree See enclosed manual Safety Notes Supply unit ebqAmbient temperature 15-35C Relative humidity Ambient temperature 15-35COverview of the Instrument CTR5000 Control PanelsComputer Interface Software Tools13 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeRemove all moving or loose parts Installation locationTransport Assembling the Microscope AssemblyThis case, read Chapter Optional accessories → p Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screws200 hrs Please regard the data sheets of the burnersType High-pressure mercury burner alternating current 100 hrsXe 75 burner Install the burner in mirror image fashionHg 50 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerTransmitted light and incident light analyzer Assembly ICT/P transmitted light polarizerMotorized polarizer DIC Prisms Optional AccessoriesMotorized analyzer Identification Sheet for the correct positionBooster Lens / Excitation Manager Assembly ErgomoduleMirror Housing Connection to the Power SupplyConnection to the CTR5000 Electronics Box Functional Principle Switching on the MicroscopeStartup StartupCombination mode Phase contrast Transmitted lightSwitch through all transmitted light processes Switch through fluorescence cubes in clockwise fashionFunction key Meaning Display Leica DM4000 B/DM4000 M Function Keys Light IntensityDiaphragms Contrast Method MagnificationKöhler Illumination Defined Function Keys on the left side of the standAbbreations are listed on p.32f Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Centering the 12 V 100 W Halogen Lamp Cube. See → p Turn the reflector into the light pathNever look directly into the light path Centering the Hg 50 W mercury lamp Focus the direct filament image with the col- lectorTurn off the microscope before exchanging Reflector cubeMercury lamps Focus the direct image with the collectorCentering the Hg 100 W and Xe 75 W Startup Startup Operation Torque adjustmentOperation Operation Rotating the Stage FocusingOperation Tubes Adjusting the Viewing DistanceAdjusting the Viewing Angle Adjusting the Eyepiece Extension to the Arm LengthEyepieces with Inlaid Reticle Correction for Vision ProblemsOperation Beam Splitting in Photo Tubes EyepiecesOperation Objectives Follow safety instructions for immersion oilStand Operation For lockable immersion objectivesFor objectives with corrective mounts Magnification ChangerOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BPolarization TL Mechanical procedureMotorized procedure Combined procedureDifferential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Illumination Trouble ShootingProblem Cause/Remedy Stand → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Handling Acids and Bases Cleaning Glass SurfacesCleaning Objectives Removing Immersion OilWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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