Leica DM4000M, DM4000B manual Index

Page 68

14. Index

14. Index

Adjusting the light sources 41 Allowable ambient conditions 15 Ambient temperature 8, 9 Ambient conditions 15 Analyzer 28, 55, 56, 59 Analyzer cube 55, 56

Aperture diaphragm 12, 35, 38, 52

Beam splitting 49

Bright field 53, 58

Booster Lens 29, 57

Cleaning 64

Cleaning objectives 65 Coaxial pinion 46 Condenser connector 18 Condenser 11, 12, 18 Condenser centering 38 Condenser height adjuster 17, 18 Condenser holder 18 Connection to power supply 30

Dark field 54, 58 Dark field stop 54 Defined function keys 36 DICprisms 28

Differentiated interference contrast 56 Display 12, 35

DMControl 11, 14

Electrical safety 8

Electronics box Leica CTR5000 8, 30 Ergomodule 29

Excitation Manager 10, 29, 57 Eyepiece 12, 49

Field diaphragm 12, 35, 52 Filter block exchanger 13 Filter cube 26

Filter cube ICR 59, 60

FIM (Fluo Intensity Manager) 10, 52

Fluorescence 57

Fluorescence intensity 57

Focus finder 33

Focusing 37, 47

Focusing telescope 39

Function keys 12, 31, 36

Gas discharge lamps 23, 24

Halogen lamp 21, 22, 42

Hg 50 burner 24

ICT/P transmitted light polarizator 27 Identification Sheet 19, 26, 28, 53 Imaging procedure10, 53, 58 Immersion oil 50, 65

Incident light axis 10

Incident light nosepiece disc 26 Incident light polarizators 27 Incident light shutter 35 Initialization 34 Interference contrast 60 Intermediate systems 16

Köhler illumination 18, 37

Lamp housing 13

Lamp housing 106 z 21, 41 Lamp housing 107/2 20, 41

Lamp housing receptacle 20, 22, 25 Light intensity 35

Light sources 41, 52

Light sources (incident light axis) 21 Light sources (transmitted light axis) 20

Magnification changer 11, 51 Mercury lamp Hg 50 W 43 Mercury lamp Hg 100W /Xe 75W 44 Mirror housing 29

Motorized analyzer 28 Motorized polarizer 27

Objective aperture 54

Objective prism slide 60

Objectives 19, 50

Objective turret 10, 12

Object stage 17, 46

Phase contrast 39, 53

Phase contrast rings (checking) 39 Polarization 55, 59

Polarizer 55, 56, 59

Polarizer L/ICR 27

Polarizer R/ICR 27

Polarizer R/P 27 Prism slot 60

Reflector cube 26

Reflector cube for lamp adjustment 41 Reset function 31

Retention pin 26 Rotating polarizer 27

Shutter 57

Software Leica DMControl 11, 14 Specimen holder 17

Supply unit ebq 100 9, 25, 46 Switch transmitted/incident light 12

Touchscreen 11 Transmitted light and incident light analyzer 28 Transmitted light axis 10 Transmitted light filter 13 Transmitted light shutter 35

Variable function keys 12, 13, 31, 36 Vision problems 49

Xe 75 burner 24

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Text symbols and their meanings → p Important Notes about this ManualImportant Notes about this Manual Explanatory noteSafety Notes Safety NotesGeneral Safety Notes MicroscopeSafety Notes Supply unit ebq Ambient temperature 15-35C Relative humidityAmbient temperature 15-35C Overvoltage category Pollution degree See enclosed manualOverview of the Instrument Control Panels Computer InterfaceSoftware Tools CTR500013 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeRemove all moving or loose parts Installation locationTransport Assembling the Microscope AssemblyThis case, read Chapter Optional accessories → p Assembly Stage Assembling the stage Stage clamp Dovetail guideAssembly Condenser Connect the condenser over the connection 8.1 with the standAssembly Tube and Eyepieces Objectives107/2 Lamp Housing Remove the lamp’s dust coverLamp housing Close the lamp housing and retighten the fas- tening screws 18.4Please regard the data sheets of the burners TypeHigh-pressure mercury burner alternating current 100 hrs 200 hrsXe 75 burner Install the burner in mirror image fashionHg 50 burner Connect the lamp housing to the power supply Rear side of the ebq 100 supply unit Lamp connectionPolarizer and Analyzer Equipping the Incident Light filter turretTransmitted light and incident light analyzer Assembly ICT/P transmitted light polarizerMotorized polarizer Optional Accessories Motorized analyzerIdentification Sheet for the correct position DIC PrismsAssembly Ergomodule Mirror HousingConnection to the Power Supply Booster Lens / Excitation ManagerConnection to the CTR5000 Electronics Box Switching on the Microscope StartupStartup Functional PrinciplePhase contrast Transmitted light Switch through all transmitted light processesSwitch through fluorescence cubes in clockwise fashion Combination modeFunction key Meaning Display Leica DM4000 B/DM4000 M Light Intensity Diaphragms Contrast MethodMagnification Function KeysKöhler Illumination Defined Function Keys on the left side of the standAbbreations are listed on p.32f Set the light intensity using the INT keys Insert the specimen in the stage’s specimen holderChecking Phase Contrast Rings Köhler IlluminationPhlr Light ring centering Clamping screw Centering the 12 V 100 W Halogen Lamp Cube. See → p Turn the reflector into the light pathNever look directly into the light path Focus the direct filament image with the col- lector Turn off the microscope before exchangingReflector cube Centering the Hg 50 W mercury lampMercury lamps Focus the direct image with the collectorCentering the Hg 100 W and Xe 75 W Startup Startup Operation Torque adjustmentOperation Focusing Operation Rotating the StageAdjusting the Viewing Distance Adjusting the Viewing AngleAdjusting the Eyepiece Extension to the Arm Length Operation TubesCorrection for Vision Problems Operation Beam Splitting in Photo TubesEyepieces Eyepieces with Inlaid ReticleFollow safety instructions for immersion oil Operation ObjectivesOperation For lockable immersion objectives For objectives with corrective mountsMagnification Changer StandOperation Light Sources Imaging Procedure Phase ContrastImaging Procedure for Leica DM4000 B/DM5000 B Dark Field TLMechanical procedure Motorized procedureCombined procedure Polarization TLDifferential Interference Contrast TL Fluorescence Cube or CubeImaging Procedure for Leica DM4000 M Dark FieldAutomatic procedure PolarizationImaging Procedure for Leica DM4000 M Interference Contrast Illumination Trouble ShootingProblem Cause/Remedy Stand Trouble Shooting Problem Cause/Remedy Bright Field → pProblem Cause/Remedy Polarization DisplayCare of the Microscope Cleaning Glass Surfaces Cleaning ObjectivesRemoving Immersion Oil Handling Acids and BasesEssential Wear and Spare Parts Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.