Leica DM4000B, DM4000M manual Polarization, Automatic procedure

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9. Imaging Procedure for Leica DM4000 M

9.1.3 Polarization

•Switch to the incident light axis (IL) by pushing the TL/IL button.

•Select the POL (polarization) contrast method. Do so by pressing the POL variable key. Alternatively: Press the CHANGE RL variable key.

(For key occupation please see “Identification Sheet”.)

The display indicates POL.

Automatic procedure:

•The ICR filter cube is automatically brought into the light path.

Mechanical procedure:

•Rotate the appropriate polarizer (71.3) and the IC/P analyzer (72.1) on the stand manually into the light path. Also bring the polarizer and analyzer into cross position until they reach maximum darkness.

•Insert a specimen and rotate a suitable objec- tive into place.

Fig. 71 Objective prism slide

1Knurled wheel for fine focusing

2	Prism slot with inserted objective prism slide	Fig. 72
3	Insert polarizer	1 Insert analyzer

1				2

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Explanatory note Text symbols and their meanings → pImportant Notes about this Manual Important Notes about this ManualMicroscope Safety NotesSafety Notes General Safety NotesOvervoltage category Pollution degree See enclosed manual Safety Notes Supply unit ebqAmbient temperature 15-35C Relative humidity Ambient temperature 15-35COverview of the Instrument CTR5000 Control PanelsComputer Interface Software Tools13 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeRemove all moving or loose parts Installation locationTransport Assembling the Microscope AssemblyThis case, read Chapter Optional accessories → p Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screws200 hrs Please regard the data sheets of the burnersType High-pressure mercury burner alternating current 100 hrsXe 75 burner Install the burner in mirror image fashionHg 50 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerTransmitted light and incident light analyzer Assembly ICT/P transmitted light polarizerMotorized polarizer DIC Prisms Optional AccessoriesMotorized analyzer Identification Sheet for the correct positionBooster Lens / Excitation Manager Assembly ErgomoduleMirror Housing Connection to the Power SupplyConnection to the CTR5000 Electronics Box Functional Principle Switching on the MicroscopeStartup StartupCombination mode Phase contrast Transmitted lightSwitch through all transmitted light processes Switch through fluorescence cubes in clockwise fashionFunction key Meaning Display Leica DM4000 B/DM4000 M Function Keys Light IntensityDiaphragms Contrast Method MagnificationKöhler Illumination Defined Function Keys on the left side of the standAbbreations are listed on p.32f Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Centering the 12 V 100 W Halogen Lamp Cube. See → p Turn the reflector into the light pathNever look directly into the light path Centering the Hg 50 W mercury lamp Focus the direct filament image with the col- lectorTurn off the microscope before exchanging Reflector cubeMercury lamps Focus the direct image with the collectorCentering the Hg 100 W and Xe 75 W Startup Startup Operation Torque adjustmentOperation Operation Rotating the Stage FocusingOperation Tubes Adjusting the Viewing DistanceAdjusting the Viewing Angle Adjusting the Eyepiece Extension to the Arm LengthEyepieces with Inlaid Reticle Correction for Vision ProblemsOperation Beam Splitting in Photo Tubes EyepiecesOperation Objectives Follow safety instructions for immersion oilStand Operation For lockable immersion objectivesFor objectives with corrective mounts Magnification ChangerOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BPolarization TL Mechanical procedureMotorized procedure Combined procedure Differential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Illumination Trouble ShootingProblem Cause/Remedy Stand → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Handling Acids and Bases Cleaning Glass SurfacesCleaning Objectives Removing Immersion OilWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity

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