Leica DM4000M, DM4000B manual Operation Light Sources

Page 52

7. Operation

7.8 Light Sources

•The brightness is set using the function keys (65.5). Then, the INT function keys are as- signed to the currently active axis for trans- mitted light (TL) or incident light (IL).

•For TL and IL:

Settings can be made either in large or small increments. Pushing both INT buttons simultaneously switches between coarse and fine setting. The display indicator changes ac- cordingly.

7.9 Aperture Diaphragm and Field Diaphragm

Both diaphragms are already factory-set to the optimum setting for the current objective.

•The AP (65.2) keys for the aperture diaphragm and the FD keys (65.4) for the field diaphragm may be used to change each diaphragm’s set- ting at any time.

Then, the function keys are assigned to the currently active axis for transmitted light (TL) or incident light (IL).

Coarse adjustment:

Fine adjustment:

0-20

======

0-255

----------

Caution:

When doing so, old values are overwritten and the new values are stored!

•For Fluo:

The brightness is set in 5 fixed steps (FIM): 100% / 55% / 35% / 20% / 10%

Caution:

While using PH or DF the aperture diaphragm is completely opened and locked.

Fig. 645 Control panel

1Variable function keys

2Aperture diaphragm

3Transmitted light/incident light

4Field diaphragm

5Light intensity

1

2

3

4

5

52

Page 51 Page 53
Image 52
Page 51 Page 53
Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Text symbols and their meanings → p Important Notes about this ManualImportant Notes about this Manual Explanatory noteSafety Notes Safety NotesGeneral Safety Notes MicroscopeSafety Notes Supply unit ebq Ambient temperature 15-35C Relative humidityAmbient temperature 15-35C Overvoltage category Pollution degree See enclosed manualOverview of the Instrument Control Panels Computer InterfaceSoftware Tools CTR500013 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeTransport Installation locationRemove all moving or loose parts This case, read Chapter Optional accessories → p AssemblyAssembling the Microscope Assembly Stage Assembling the stage Stage clamp Dovetail guideAssembly Condenser Connect the condenser over the connection 8.1 with the standAssembly Tube and Eyepieces Objectives107/2 Lamp Housing Remove the lamp’s dust coverLamp housing Close the lamp housing and retighten the fas- tening screws 18.4Please regard the data sheets of the burners TypeHigh-pressure mercury burner alternating current 100 hrs 200 hrsHg 50 burner Install the burner in mirror image fashionXe 75 burner Connect the lamp housing to the power supply Rear side of the ebq 100 supply unit Lamp connectionPolarizer and Analyzer Equipping the Incident Light filter turretMotorized polarizer Assembly ICT/P transmitted light polarizerTransmitted light and incident light analyzer Optional Accessories Motorized analyzerIdentification Sheet for the correct position DIC PrismsAssembly Ergomodule Mirror HousingConnection to the Power Supply Booster Lens / Excitation ManagerConnection to the CTR5000 Electronics Box Switching on the Microscope StartupStartup Functional PrinciplePhase contrast Transmitted light Switch through all transmitted light processesSwitch through fluorescence cubes in clockwise fashion Combination modeFunction key Meaning Display Leica DM4000 B/DM4000 M Light Intensity Diaphragms Contrast MethodMagnification Function KeysAbbreations are listed on p.32f Defined Function Keys on the left side of the standKöhler Illumination Set the light intensity using the INT keys Insert the specimen in the stage’s specimen holderChecking Phase Contrast Rings Köhler IlluminationPhlr Light ring centering Clamping screw Never look directly into the light path Cube. See → p Turn the reflector into the light pathCentering the 12 V 100 W Halogen Lamp Focus the direct filament image with the col- lector Turn off the microscope before exchangingReflector cube Centering the Hg 50 W mercury lampCentering the Hg 100 W and Xe 75 W Focus the direct image with the collectorMercury lamps Startup Startup Operation Torque adjustmentOperation Focusing Operation Rotating the StageAdjusting the Viewing Distance Adjusting the Viewing AngleAdjusting the Eyepiece Extension to the Arm Length Operation TubesCorrection for Vision Problems Operation Beam Splitting in Photo TubesEyepieces Eyepieces with Inlaid ReticleFollow safety instructions for immersion oil Operation ObjectivesOperation For lockable immersion objectives For objectives with corrective mountsMagnification Changer StandOperation Light Sources Imaging Procedure Phase ContrastImaging Procedure for Leica DM4000 B/DM5000 B Dark Field TLMechanical procedure Motorized procedureCombined procedure Polarization TLDifferential Interference Contrast TL Fluorescence Cube or CubeImaging Procedure for Leica DM4000 M Dark FieldAutomatic procedure PolarizationImaging Procedure for Leica DM4000 M Interference Contrast Problem Cause/Remedy Stand Trouble ShootingIllumination Trouble Shooting Problem Cause/Remedy Bright Field → pProblem Cause/Remedy Polarization DisplayCare of the Microscope Cleaning Glass Surfaces Cleaning ObjectivesRemoving Immersion Oil Handling Acids and BasesEssential Wear and Spare Parts Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
Related manuals
Manual 164 pages 54.45 Kb

DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
Top Page Image Contents