Leica DM4000B manual Focus the direct image with the collector, Centering the Hg 100 W and Xe 75 W

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6. Startup

•Focus the direct image with the collector (46.6).

•Use the adjusting buttons on the rear side of the lamp housing (46.2,46.4) to pivot the arc’s mirror image to the side or completely out of the beam path. The lamp filament’s focused image remains visible (Fig. 50).

•Use the adjusting buttons (46.1) and (46.5) to place the direct arc image right or left on an imaginary center line of the centering plane (Fig. 51).

•Then pivot the arc’s mirror image with the ad- justing knobs (46.2 and 4) and focus it using the reflector (46.3).

•Use the adjusting knobs (46.2 and 4) to orient the mirror image symmetrically to the direct image (Fig. 52).

•Defocus the image with the collector knob (46.6) until the arc image and mirror image are no longer recognizable and the image is uniformly illuminated.

•Exchange the reflector cube for lamp adjustment for the original filter cube.

Fig. 50 Direct arc image focused but decentered (in reality, the image is less focused)

Fig. 51 Direct arc image in target position (in reality, the image is less focused)

Centering the Hg 100 W and Xe 75 W

Fig. 52 Direct arc image and mirror image in target

position (in reality, the image is less focused)

mercury lamps

 

•In the adjustment window, you see the direct arc image and the mirror image, which in most cases are shifted together.

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Explanatory note Text symbols and their meanings → pImportant Notes about this Manual Important Notes about this ManualMicroscope Safety NotesSafety Notes General Safety NotesOvervoltage category Pollution degree See enclosed manual Safety Notes Supply unit ebqAmbient temperature 15-35C Relative humidity Ambient temperature 15-35COverview of the Instrument CTR5000 Control PanelsComputer Interface Software Tools13 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeTransport Installation locationRemove all moving or loose parts This case, read Chapter Optional accessories → p AssemblyAssembling the Microscope Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screws200 hrs Please regard the data sheets of the burnersType High-pressure mercury burner alternating current 100 hrsHg 50 burner Install the burner in mirror image fashionXe 75 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerMotorized polarizer Assembly ICT/P transmitted light polarizerTransmitted light and incident light analyzer DIC Prisms Optional AccessoriesMotorized analyzer Identification Sheet for the correct positionBooster Lens / Excitation Manager Assembly ErgomoduleMirror Housing Connection to the Power SupplyConnection to the CTR5000 Electronics Box Functional Principle Switching on the MicroscopeStartup StartupCombination mode Phase contrast Transmitted lightSwitch through all transmitted light processes Switch through fluorescence cubes in clockwise fashionFunction key Meaning Display Leica DM4000 B/DM4000 M Function Keys Light IntensityDiaphragms Contrast Method MagnificationAbbreations are listed on p.32f Defined Function Keys on the left side of the standKöhler Illumination Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Never look directly into the light path Cube. See → p Turn the reflector into the light pathCentering the 12 V 100 W Halogen Lamp Centering the Hg 50 W mercury lamp Focus the direct filament image with the col- lectorTurn off the microscope before exchanging Reflector cubeCentering the Hg 100 W and Xe 75 W Focus the direct image with the collectorMercury lamps Startup Startup Operation Torque adjustmentOperation Operation Rotating the Stage FocusingOperation Tubes Adjusting the Viewing DistanceAdjusting the Viewing Angle Adjusting the Eyepiece Extension to the Arm LengthEyepieces with Inlaid Reticle Correction for Vision ProblemsOperation Beam Splitting in Photo Tubes EyepiecesOperation Objectives Follow safety instructions for immersion oilStand Operation For lockable immersion objectivesFor objectives with corrective mounts Magnification ChangerOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BPolarization TL Mechanical procedureMotorized procedure Combined procedureDifferential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Problem Cause/Remedy Stand Trouble ShootingIllumination → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Handling Acids and Bases Cleaning Glass SurfacesCleaning Objectives Removing Immersion OilWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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