Leica DM4000B, DM4000M Operation Beam Splitting in Photo Tubes, Eyepieces with Inlaid Reticle

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7. Operation

Beam Splitting in Photo Tubes

EDT22 tube:

The beam splitting between the observation and documentation outputs has a definite presetting (50:50).

BDT25+ tube:

The beam splitting is set manually by pulling out a control bar.

Control Bar

 

 

 

 

Observation

Photo

VIS

 

 

 

 

100 %

0 %

 

 

 

 

50/50

 

 

 

50 %

50 %

 

 

 

 

 

 

 

PHOTO

 

 

0 %

100 %

 

 

 

 

 

 

 

 

MBDT25+ tube:

This tube is similar to the documentation tube BDT25+, but it is motorized.

The control positions are selected using a vari- able function key on the stand.

HC L 2TU tube:

The beam splitting is set manually by pulling out a control bar.

Control Bar

 

 

 

 

Observation

Photo

VIS

 

 

 

 

100 %

0 %

PHOTO

 

 

 

 

0 %

100 %

 

 

 

 

7.5 Eyepieces

Note:

The eyepiece’s aperture protector must be removed,or at least folded back, during microscopy while wearing eyeglasses.

Eyeglasses with multifocal lenses (bifocals and smooth view glasses) must be removed while operating the microscope.

•For the adjustable tubes with documentation output, choose the 100% position.

Eyepieces with Inlaid Reticle

•Focus the reticle by adjusting the eyelens.

•Focus on the object through this eyepiece.

•Then, close that eye and focus on the specimen by adjusting only the second ocular.

Correction for Vision Problems

Fig. 62 BDT25+ tube with digital camera

1Control bar

•With your right eye, look through the right eyepiece and bring the specimen into sharp focus.

•Then, with your left eye, view the same speci- men and rotate the left eyepiece tube until the object is brought into sharp focus. Do not use the focus dial.

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Important Notes about this Manual Text symbols and their meanings → pImportant Notes about this Manual Explanatory noteSafety Notes Safety NotesGeneral Safety Notes MicroscopeAmbient temperature 15-35C Relative humidity Safety Notes Supply unit ebqAmbient temperature 15-35C Overvoltage category Pollution degree See enclosed manualOverview of the Instrument Computer Interface Control PanelsSoftware Tools CTR500013 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeTransport Installation locationRemove all moving or loose parts This case, read Chapter Optional accessories → p AssemblyAssembling the Microscope Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screwsType Please regard the data sheets of the burnersHigh-pressure mercury burner alternating current 100 hrs 200 hrsHg 50 burner Install the burner in mirror image fashionXe 75 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerMotorized polarizer Assembly ICT/P transmitted light polarizerTransmitted light and incident light analyzer Motorized analyzer Optional AccessoriesIdentification Sheet for the correct position DIC PrismsMirror Housing Assembly ErgomoduleConnection to the Power Supply Booster Lens / Excitation ManagerConnection to the CTR5000 Electronics Box Startup Switching on the MicroscopeStartup Functional PrincipleSwitch through all transmitted light processes Phase contrast Transmitted lightSwitch through fluorescence cubes in clockwise fashion Combination modeFunction key Meaning Display Leica DM4000 B/DM4000 M Diaphragms Contrast Method Light IntensityMagnification Function KeysAbbreations are listed on p.32f Defined Function Keys on the left side of the standKöhler Illumination Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Never look directly into the light path Cube. See → p Turn the reflector into the light pathCentering the 12 V 100 W Halogen Lamp Turn off the microscope before exchanging Focus the direct filament image with the col- lectorReflector cube Centering the Hg 50 W mercury lampCentering the Hg 100 W and Xe 75 W Focus the direct image with the collectorMercury lamps Startup Startup Operation Torque adjustment Operation Operation Rotating the Stage FocusingAdjusting the Viewing Angle Adjusting the Viewing DistanceAdjusting the Eyepiece Extension to the Arm Length Operation TubesOperation Beam Splitting in Photo Tubes Correction for Vision ProblemsEyepieces Eyepieces with Inlaid ReticleOperation Objectives Follow safety instructions for immersion oilFor objectives with corrective mounts Operation For lockable immersion objectivesMagnification Changer StandOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BMotorized procedure Mechanical procedureCombined procedure Polarization TLDifferential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Problem Cause/Remedy Stand Trouble ShootingIllumination → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Cleaning Objectives Cleaning Glass SurfacesRemoving Immersion Oil Handling Acids and BasesWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
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