Leica DM4000B, DM4000M manual Function key Meaning

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6. Startup

For Leica DM4000 M:
Function key		Meaning

BF		Bright field (Incident light)
ICR		Interference contrast (Incident light)
DF		Dark field (Incident light)
POL		Polarization (Incident light)
CHANGE RL		Switch through all incident light processes
INT	↑	Increase brightness (incident light)
INT	↓	Reduce brightness (incident light)
AP	↑	Open aperture diaphragm (incident light)
AP	↓	Close aperture diaphragm (incident light)
FD	↑	Open field diaphragm (incident light)
FD	↓	Close field diaphragm (incident light)
SHUTTER RL		Open/close incident light shutter
FLUO		Fluorescence (last filter cube)
CUBE 1		Select fluorescence cube at position 1
CHANGE FLUO		Switch through fluorescence cubes
FOCUS FINDER		Select smallest field diaphragm and switch back to original field
		diaphragm by pressing the key again
BF TL		Bright field (Transmitted light )
INT	↑	Increase brightness (transmitted light)
INT	↓	Reduce brightness (transmitted light)
AP	↑	Open aperture diaphragm (transmitted light)
AP	↓	Close aperture diaphragm (transmitted light)
FD	↑	Open field diaphragm (transmitted light)
FD	↓	Close field diaphragm (transmitted light)
COMBI		Combination process (BF and BF TL)

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Important Notes about this Manual Text symbols and their meanings → pImportant Notes about this Manual Explanatory noteSafety Notes Safety NotesGeneral Safety Notes MicroscopeAmbient temperature 15-35C Relative humidity Safety Notes Supply unit ebqAmbient temperature 15-35C Overvoltage category Pollution degree See enclosed manualOverview of the Instrument Computer Interface Control PanelsSoftware Tools CTR500013 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeInstallation location TransportRemove all moving or loose parts Assembly This case, read Chapter Optional accessories → pAssembling the Microscope Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screwsType Please regard the data sheets of the burnersHigh-pressure mercury burner alternating current 100 hrs 200 hrsInstall the burner in mirror image fashion Hg 50 burnerXe 75 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerAssembly ICT/P transmitted light polarizer Motorized polarizerTransmitted light and incident light analyzer Motorized analyzer Optional AccessoriesIdentification Sheet for the correct position DIC PrismsMirror Housing Assembly ErgomoduleConnection to the Power Supply Booster Lens / Excitation Manager Connection to the CTR5000 Electronics Box Startup Switching on the MicroscopeStartup Functional PrincipleSwitch through all transmitted light processes Phase contrast Transmitted lightSwitch through fluorescence cubes in clockwise fashion Combination modeFunction key Meaning Display Leica DM4000 B/DM4000 M Diaphragms Contrast Method Light IntensityMagnification Function KeysDefined Function Keys on the left side of the stand Abbreations are listed on p.32fKöhler Illumination Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Cube. See → p Turn the reflector into the light path Never look directly into the light pathCentering the 12 V 100 W Halogen Lamp Turn off the microscope before exchanging Focus the direct filament image with the col- lectorReflector cube Centering the Hg 50 W mercury lampFocus the direct image with the collector Centering the Hg 100 W and Xe 75 WMercury lamps Startup Startup Torque adjustment OperationOperation Operation Rotating the Stage FocusingAdjusting the Viewing Angle Adjusting the Viewing DistanceAdjusting the Eyepiece Extension to the Arm Length Operation TubesOperation Beam Splitting in Photo Tubes Correction for Vision ProblemsEyepieces Eyepieces with Inlaid ReticleOperation Objectives Follow safety instructions for immersion oilFor objectives with corrective mounts Operation For lockable immersion objectivesMagnification Changer StandOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BMotorized procedure Mechanical procedureCombined procedure Polarization TLDifferential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Trouble Shooting Problem Cause/Remedy StandIllumination → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Cleaning Objectives Cleaning Glass SurfacesRemoving Immersion Oil Handling Acids and BasesWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity

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