Leica DM4000B, DM4000M manual Fluorescence, Cube or Cube

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8. Imaging Procedure for Leica DM4000 B/DM5000 B

8.2 Fluorescence

•Switch to the fluorescent light axis (FLUO) by pushing the TL/IL button.

•Insert a specimen and rotate a suitable objec- tive into place.

•The current fluorescence cube is indicated on the display.

•Closing the incident light shutter protects your specimen from fading.

Do so by pressing the SHUTTER variable key. (For key occupation please see “Identification Sheet”.)

The display indicates the symbol: ↓

•Selecting the fluorescence filter cube: Press the variable keys

Cube or Cube

•The fluorescence intensity can be increased using the Booster Lens on the right side of the stand (Fig. 69).

•For multifluorescence, use of a Excitation Manager is recommended. The Excitation Manager is inserted into the right side of the stand up to the last stop (Fig. 70).

•Using Booster Lens and Excitation Manager, the Excitation Manager can be inserted into the back receptacle.

Fig. 69 Inserting the Booster Lens

Fig. 70 Inserting the Excitation Manager

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Important Notes about this Manual Text symbols and their meanings → pImportant Notes about this Manual Explanatory noteSafety Notes Safety NotesGeneral Safety Notes MicroscopeAmbient temperature 15-35C Relative humidity Safety Notes Supply unit ebqAmbient temperature 15-35C Overvoltage category Pollution degree See enclosed manualOverview of the Instrument Computer Interface Control PanelsSoftware Tools CTR500013 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeInstallation location TransportRemove all moving or loose parts Assembly This case, read Chapter Optional accessories → pAssembling the Microscope Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screwsType Please regard the data sheets of the burnersHigh-pressure mercury burner alternating current 100 hrs 200 hrsInstall the burner in mirror image fashion Hg 50 burnerXe 75 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerAssembly ICT/P transmitted light polarizer Motorized polarizerTransmitted light and incident light analyzer Motorized analyzer Optional AccessoriesIdentification Sheet for the correct position DIC PrismsMirror Housing Assembly ErgomoduleConnection to the Power Supply Booster Lens / Excitation ManagerConnection to the CTR5000 Electronics Box Startup Switching on the MicroscopeStartup Functional PrincipleSwitch through all transmitted light processes Phase contrast Transmitted lightSwitch through fluorescence cubes in clockwise fashion Combination modeFunction key Meaning Display Leica DM4000 B/DM4000 M Diaphragms Contrast Method Light IntensityMagnification Function KeysDefined Function Keys on the left side of the stand Abbreations are listed on p.32fKöhler Illumination Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Cube. See → p Turn the reflector into the light path Never look directly into the light pathCentering the 12 V 100 W Halogen Lamp Turn off the microscope before exchanging Focus the direct filament image with the col- lectorReflector cube Centering the Hg 50 W mercury lampFocus the direct image with the collector Centering the Hg 100 W and Xe 75 WMercury lamps Startup Startup Torque adjustment OperationOperation Operation Rotating the Stage FocusingAdjusting the Viewing Angle Adjusting the Viewing DistanceAdjusting the Eyepiece Extension to the Arm Length Operation TubesOperation Beam Splitting in Photo Tubes Correction for Vision ProblemsEyepieces Eyepieces with Inlaid ReticleOperation Objectives Follow safety instructions for immersion oilFor objectives with corrective mounts Operation For lockable immersion objectivesMagnification Changer StandOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BMotorized procedure Mechanical procedureCombined procedure Polarization TLDifferential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Trouble Shooting Problem Cause/Remedy StandIllumination → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Cleaning Objectives Cleaning Glass SurfacesRemoving Immersion Oil Handling Acids and BasesWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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