Leica DM4000M, DM4000B manual Trouble Shooting Problem Cause/Remedy Bright Field, → p

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10. Trouble Shooting

Problem

Cause/Remedy

 

 

Bright Field

The specimen can not be brought into focus.

Use the correct immersion medium.

Lay the specimen with the cover glass to- wards the top.

Make sure that the cover glass thickness is correct and that is conform to the indication on the objective.

Check the condenser centering.

Dark Field

No definite DF contrast is possible.

Be sure that a DF objective is being used.

The objective aperture setting is too high (maximum 0.75). If necessary, reduce the ob- jective aperture using the iris diaphragm on the objective.

Check the condenser centering.

The image is unevenly or not uniformly illumi- nated.

The magnification is too weak. Use a higher magnification.

Undesirable stray light

Clean the specimen and neighboring lenses

 

(→ p. 65).

Phase contrast

No phase contrast is possible.

The specimen is too thick.

The refraction index of embedding material and object is identical.

The cover glass is not placed evenly. Therefore, check the centering of the light rings (→ p. 39).

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Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Important Notes about this Manual Text symbols and their meanings → pImportant Notes about this Manual Explanatory noteGeneral Safety Notes Safety NotesSafety Notes MicroscopeAmbient temperature 15-35C Safety Notes Supply unit ebqAmbient temperature 15-35C Relative humidity Overvoltage category Pollution degree See enclosed manualOverview of the Instrument Software Tools Control PanelsComputer Interface CTR500013 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeRemove all moving or loose parts Installation locationTransport Assembling the Microscope AssemblyThis case, read Chapter Optional accessories → p Assembly Stage Assembling the stage Stage clamp Dovetail guideAssembly Condenser Connect the condenser over the connection 8.1 with the standAssembly Tube and Eyepieces Objectives107/2 Lamp Housing Remove the lamp’s dust coverLamp housing Close the lamp housing and retighten the fas- tening screws 18.4High-pressure mercury burner alternating current 100 hrs Please regard the data sheets of the burnersType 200 hrsXe 75 burner Install the burner in mirror image fashionHg 50 burner Connect the lamp housing to the power supply Rear side of the ebq 100 supply unit Lamp connectionPolarizer and Analyzer Equipping the Incident Light filter turretTransmitted light and incident light analyzer Assembly ICT/P transmitted light polarizerMotorized polarizer Identification Sheet for the correct position Optional AccessoriesMotorized analyzer DIC PrismsConnection to the Power Supply Assembly ErgomoduleMirror Housing Booster Lens / Excitation ManagerConnection to the CTR5000 Electronics Box Startup Switching on the MicroscopeStartup Functional PrincipleSwitch through fluorescence cubes in clockwise fashion Phase contrast Transmitted lightSwitch through all transmitted light processes Combination modeFunction key Meaning Display Leica DM4000 B/DM4000 M Magnification Light IntensityDiaphragms Contrast Method Function KeysKöhler Illumination Defined Function Keys on the left side of the standAbbreations are listed on p.32f Set the light intensity using the INT keys Insert the specimen in the stage’s specimen holderChecking Phase Contrast Rings Köhler IlluminationPhlr Light ring centering Clamping screw Centering the 12 V 100 W Halogen Lamp Cube. See → p Turn the reflector into the light pathNever look directly into the light path Reflector cube Focus the direct filament image with the col- lectorTurn off the microscope before exchanging Centering the Hg 50 W mercury lampMercury lamps Focus the direct image with the collectorCentering the Hg 100 W and Xe 75 W Startup Startup Operation Torque adjustmentOperation Focusing Operation Rotating the StageAdjusting the Eyepiece Extension to the Arm Length Adjusting the Viewing DistanceAdjusting the Viewing Angle Operation TubesEyepieces Correction for Vision ProblemsOperation Beam Splitting in Photo Tubes Eyepieces with Inlaid ReticleFollow safety instructions for immersion oil Operation ObjectivesMagnification Changer Operation For lockable immersion objectivesFor objectives with corrective mounts StandOperation Light Sources Imaging Procedure Phase ContrastImaging Procedure for Leica DM4000 B/DM5000 B Dark Field TLCombined procedure Mechanical procedureMotorized procedure Polarization TLDifferential Interference Contrast TL Fluorescence Cube or CubeImaging Procedure for Leica DM4000 M Dark FieldAutomatic procedure PolarizationImaging Procedure for Leica DM4000 M Interference Contrast Illumination Trouble ShootingProblem Cause/Remedy Stand Trouble Shooting Problem Cause/Remedy Bright Field → pProblem Cause/Remedy Polarization DisplayCare of the Microscope Removing Immersion Oil Cleaning Glass SurfacesCleaning Objectives Handling Acids and BasesEssential Wear and Spare Parts Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity
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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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