Leica DM4000B, DM4000M manual Problem Cause/Remedy Polarization, Display

Page 63

10. Trouble Shooting

Problem	Cause/Remedy

Polarization

No polarization contrast is possible.

Bring the polarizer and analyzer into cross po- sition until they reach maximum darkness (without specimen) (→ p. 55, 59).

Fluorescence

The image is completely dark (no fluorescence).

Open the shutter (→ p. 57).

Select the incident light axis (IL) (→ p. 36). Check the antigen-antibody combination.

The fluorescence is too weak.

Insert the Booster Lens (→ p. 29).

Center the lamp (→	p. 41ff).
Insert a new lamp (→	p. 20f).

Display

The display flashes.	Rotate an appropriate objective for the con-
	trast method into the light path.

FAIL! appears.	Check insertion of objectives, cubes, etc.

Image 63

Contents Leica DM4000 B Page Leica DM4000 B Copyrights CopyrightsContents Imaging Procedure for Essential Explanatory note Text symbols and their meanings → pImportant Notes about this Manual Important Notes about this ManualMicroscope Safety NotesSafety Notes General Safety NotesOvervoltage category Pollution degree See enclosed manual Safety Notes Supply unit ebqAmbient temperature 15-35C Relative humidity Ambient temperature 15-35COverview of the Instrument CTR5000 Control PanelsComputer Interface Software Tools13 12 11 10 9 Overview of the Instrument Unpacking the Microscope Unpacking the MicroscopeInstallation location TransportRemove all moving or loose parts Assembly This case, read Chapter Optional accessories → pAssembling the Microscope Assembling the stage Stage clamp Dovetail guide Assembly StageConnect the condenser over the connection 8.1 with the stand Assembly CondenserObjectives Assembly Tube and EyepiecesRemove the lamp’s dust cover 107/2 Lamp HousingLamp housing 18.4 Close the lamp housing and retighten the fas- tening screws200 hrs Please regard the data sheets of the burnersType High-pressure mercury burner alternating current 100 hrsInstall the burner in mirror image fashion Hg 50 burnerXe 75 burner Rear side of the ebq 100 supply unit Lamp connection Connect the lamp housing to the power supplyEquipping the Incident Light filter turret Polarizer and AnalyzerAssembly ICT/P transmitted light polarizer Motorized polarizerTransmitted light and incident light analyzer DIC Prisms Optional AccessoriesMotorized analyzer Identification Sheet for the correct positionBooster Lens / Excitation Manager Assembly ErgomoduleMirror Housing Connection to the Power SupplyConnection to the CTR5000 Electronics Box Functional Principle Switching on the MicroscopeStartup StartupCombination mode Phase contrast Transmitted lightSwitch through all transmitted light processes Switch through fluorescence cubes in clockwise fashionFunction key Meaning Display Leica DM4000 B/DM4000 M Function Keys Light IntensityDiaphragms Contrast Method MagnificationDefined Function Keys on the left side of the stand Abbreations are listed on p.32fKöhler Illumination Insert the specimen in the stage’s specimen holder Set the light intensity using the INT keysKöhler Illumination Checking Phase Contrast RingsPhlr Light ring centering Clamping screw Cube. See → p Turn the reflector into the light path Never look directly into the light pathCentering the 12 V 100 W Halogen Lamp Centering the Hg 50 W mercury lamp Focus the direct filament image with the col- lectorTurn off the microscope before exchanging Reflector cubeFocus the direct image with the collector Centering the Hg 100 W and Xe 75 WMercury lamps Startup Startup Torque adjustment OperationOperation Operation Rotating the Stage FocusingOperation Tubes Adjusting the Viewing DistanceAdjusting the Viewing Angle Adjusting the Eyepiece Extension to the Arm LengthEyepieces with Inlaid Reticle Correction for Vision ProblemsOperation Beam Splitting in Photo Tubes EyepiecesOperation Objectives Follow safety instructions for immersion oilStand Operation For lockable immersion objectivesFor objectives with corrective mounts Magnification ChangerOperation Light Sources Phase Contrast Imaging ProcedureDark Field TL Imaging Procedure for Leica DM4000 B/DM5000 BPolarization TL Mechanical procedureMotorized procedure Combined procedureDifferential Interference Contrast TL Cube or Cube FluorescenceDark Field Imaging Procedure for Leica DM4000 MPolarization Automatic procedureImaging Procedure for Leica DM4000 M Interference Contrast Trouble Shooting Problem Cause/Remedy StandIllumination → p Trouble Shooting Problem Cause/Remedy Bright FieldDisplay Problem Cause/Remedy PolarizationCare of the Microscope Handling Acids and Bases Cleaning Glass SurfacesCleaning Objectives Removing Immersion OilWear and Spare Parts Essential Wear and Spare PartsAbbreviations and Pictograms Abbreviations and PictogramsIndex IndexEU Declaration of Conformity EU Declaration of Conformity

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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

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