Working Conditions

The thermal cycler is designed operate under the following conditions:

∙Indoor use

∙Ambient temperature range +5oC to +40oC

∙Altitude to 2000m

∙Relative humidity not exceeding 95%

∙Power supply fluctuations not exceeding 10%

∙Over voltage category II IEC 60364-4-443

∙Pollution degree 2

Note: The control specifications are quoted at an ambient temperature of 20oC. The specification may deteriorate outside an ambient temperature of 10oC to 30oC.

The instrument has been tested for radio frequency interference and is certified under EN61326.

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Omega Engineering 30, 48, 20, 25 manual Working Conditions

Contents

User’sGuide TCY 20, 25, 30Thermal Cyclers Shop online at Servicing North America OMEGAnet Online Service omega.comInternet e-mail info@omega.com For immediate technical or application assistance Contents 2. Safety and installation 1. IntroductionOperator Safety Unpacking InstallationReplacement cable ELECTRICIAN Working Conditions Heating/Cooling Rate 3. Cycler TCY SpecificationTemperature Heated Lid Power 4. User operation and consumablesDimensions Heated lid 0.5ml Tubes to select TCYTCProgramsMethods00 Information Configuration Programs Configuration5. Front panel controls Methods The Up arrow key The keysThe End, Finish or Exit key The Down arrow key 6. Programming the CYCLER The Alpha/Numeric keys Cycle MethodsPrograms00 Information ConfigurationStage Step Run ProgramMethod 2 STEP TEMPLATESTEP Option 1 Creating programs using templates To give a program a name To save the program to select DONNA 7 Run programmethod ProgramsMethods00 Information ConfigurationSet password to select To edit a field or step in an existing program 72.0ºC Post hold10.0 5m00 To set a password for a selected program UNNAMED #10.0ºCºC 105ºC New*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Example programsProgramming Typical 3-Step PCR to edit the step as To insert additional stepsTo delete a step described above To add a new stage before an existing one to insertstage To add a new stage at the end To insert a ‘pause’ into a program The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’ Press “Enter” Press “Pause” To program increment or decrement temperature or timeTemperature profile of incremented Hold Time at step 3 of each cycle to go into edit mode twice to go into edit View methodProgram Run ProgrammethodGEORGE Stopping what you are doing to select Run methodProgramNewprogramMethods To run a program Hold @ Sample58.0ºC 72.0ºC Block s/nid 8. Configuration7. Information Unit s/n PasswordsFormat memory ProgramSettingsdefaultsxxxx Settings SURE ? Thiswill delete Passwords 9. Notes on transferring protocols from other thermal cyclers Heater Block removal 10. User maintenanceCleaning your CYCLER Underside of the CYCLER TCY 11. Some frequently asked questions Insulation testing 12. Additional informationFault Finding Fuses WARRANTY/DISCLAIMER RETURN REQUESTS/INQUIRIES FLOW/LEVEL TEMPERATUREPRESSURE, STRAIN AND FORCE pH/CONDUCTIVITY