Contents

Contents
1.	Introduction	4
2.	Safety and installation	4
3.	Cycler TCY specification	7
4. User operation and consumables		8
	Heated lid	8
	Tubes or reaction vessels	8
	0.5ml Tubes	9
	Switching on	10
5.	Front panel controls	11
	The LCD Display	11
	Indicators	11
	The keys	12
6. Programming the Cycler TCY		13
	Programming Options	15
	Option 1: Creating programs using templates	19
	Option 2: Creating a new program	20
	Example programs	20
	Programming	20
	To program increment or decrement temperature or time	24
	To run a program	27
7.	Information	29
8.	Configuration	29
9.	Notes on transferring protocols from other thermal cyclers	32
10. User maintenance		33
	Cleaning your Cycler TCY	33
	Heater Block removal	33
11. Some frequently asked questions		34
12. Additional information		35
	Fault Finding	35
	Fuses	35
	The heated lid over-temperature cut-out	35
	Insulation testing	35
	Interchangeable blocks	35

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Contents

Shop online at TCY 20, 25, 30Thermal Cyclers User’sGuide For immediate technical or application assistance OMEGAnet Online Service omega.comInternet e-mail info@omega.com Servicing North America Contents 2. Safety and installation 1. IntroductionOperator Safety ELECTRICIAN InstallationReplacement cable Unpacking Working Conditions Heated Lid 3. Cycler TCY SpecificationTemperature Heating/Cooling Rate Heated lid 4. User operation and consumablesDimensions Power 0.5ml Tubes Information Configuration TCYTCProgramsMethods00 to select Methods Configuration5. Front panel controls Programs The Down arrow key The keysThe End, Finish or Exit key The Up arrow key The Alpha/Numeric keys 6. Programming the CYCLER Step MethodsPrograms00 Information ConfigurationStage Cycle Option 1 Creating programs using templates 2 STEP TEMPLATESTEP Run ProgramMethod To save the program To give a program a name To edit a field or step in an existing program ProgramsMethods00 Information ConfigurationSet password to select to select DONNA 7 Run programmethod 5m00 Post hold10.0 72.0ºC 105ºC UNNAMED #10.0ºCºC To set a password for a selected program Typical 3-Step PCR Example programsProgramming New*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE described above To insert additional stepsTo delete a step to edit the step as To add a new stage at the end to insertstage To add a new stage before an existing one The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’ To insert a ‘pause’ into a program to go into edit mode To program increment or decrement temperature or timeTemperature profile of incremented Hold Time at step 3 of each cycle Press “Enter” Press “Pause” twice to go into edit Stopping what you are doing Run ProgrammethodGEORGE View methodProgram To run a program Run methodProgramNewprogramMethods to select 72.0ºC Sample58.0ºC Hold @ Unit s/n 8. Configuration7. Information Block s/nid Settings ProgramSettingsdefaultsxxxx PasswordsFormat memory Passwords Thiswill delete SURE ? 9. Notes on transferring protocols from other thermal cyclers Underside of the CYCLER TCY 10. User maintenanceCleaning your CYCLER Heater Block removal 11. Some frequently asked questions Fuses 12. Additional informationFault Finding Insulation testing RETURN REQUESTS/INQUIRIES WARRANTY/DISCLAIMER pH/CONDUCTIVITY TEMPERATUREPRESSURE, STRAIN AND FORCE FLOW/LEVEL

Omega Engineering 25, 48, 20, 30 manual Contents

Models: 25 30 20 48