The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 20µl or less.
During the final
0.5ml Tubes
The heated lid mechanism of the Cycler TCY with the 0.5ml block is optimised for flat top tubes. In order to fit the maximum number of tubes in the block, they need to be placed so that all the hinges are in the same relative orientation. An example is illustrated below.
Place the tubes in the block in the same orientation in order to achieve the maximum capacity.
Always make sure the pressure on the heated lid is balanced by spreading the tubes across the block or inserting some dummy tubes if necessary.
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