Nikon DS-5M, DS-5MC, DS-L1 136

	.MICROSCOPE.	VI Basic Operations

6Using the Microscope Control

2)When the Nikon 80i microscope incorporating DIH-E is connected

	Parts	Menu displayed

Focus (Z axis)		No menu displayed

Nosepiece	Intelligent nosepiece	Status display menu displayed

	Manual nosepiece	No menu displayed

Optical zoom		Control/status display menu displayed

Optical path		Control/status display menu displayed

Shutter		Control/status display menu displayed

Filter cube		Control/status display menu displayed

Lamp		No menu displayed

Universal condenser		No menu displayed

Diascopic illumination aperture diaphragm		No menu displayed

Diascopic illumination field diaphragm		No menu displayed

Episcopic illumination field diaphragm		Control/status display menu displayed

ND filter		No menu displayed

Analyzer		Control/status display menu displayed

3)When the Nikon 90i microscope without DIH is connected

		Parts	Menu displayed

	Focus (Z axis)		Control/status display menu displayed

	Nosepiece	Motorized nosepiece	Control/status display menu displayed

	Optical zoom		No menu displayed

	Optical path		No menu displayed

	Shutter		No menu displayed

	Filter cube		No menu displayed

	Lamp		Control/status display menu displayed

		Motorized universal	Control/status display menu displayed
	Universal	condenser	Control/status display menu displayed
		condenser

	condenser	Non-motorized universal	No menu displayed
		Non-motorized universal
		condenser
		condenser

	Diascopic	With motorized diaphragm	Control/status display menu displayed
	illumination	With motorized diaphragm	Control/status display menu displayed
	illumination
	aperture	Without motorized	No menu displayed
	diaphragm	diaphragm	No menu displayed
	diaphragm	diaphragm

	Diascopic illumination field diaphragm		Control/status display menu displayed

	Episcopic illumination field diaphragm		No menu displayed

	ND filter	With motorized ND filter	Control/status display menu displayed

		Without motorized ND filter	No menu displayed

	Analyzer		No menu displayed

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Contents

<Instructions > (Ver. 2.2) Page Warning and Cautionary Symbols Used in This Manual SymbolMeaning Trademarks Microsoft, Windows Internet Explorer 1. Using the equipment 2. Do not disassemble or repair the equipment 3. DS AC adapter 4. Power cord •When using the product in 220-240V AC regions: 1.Installation environment 3.Connect only the devices specified to the connectors 4.Cautions on setup, installation, and storage of the equipment CONTENTS Page Page Components of the Equipment Peripheral Equipment (1) Analog RGB input display (2) USB mouse (3) USB keyboard (4) USB hub (5) USB printer (6) CF card (7) C mount lens (8)Network connection cable (10) Personal computer (PC) (11) Nikon 80i/90i Microscope Names of Parts and Their Functions Front of the DS-L1 Left side of the DS-L1 Right side of the DS-L1 Rear of the DS-L1 2DS Camera Head DS-5M /DS Cooled Camera Head DS-5Mc 3DS Remote Controller DS-RC(Optional) Connecting and Installing the Equipment Schematic diagram PC or a PictBridge supporting printer LAN Connectors · DS-L1Left side · DS-L1Rear 2Installing the DS-L1 When attaching a commercially available arm to the DS-L1 (1)Connecting the DS Camera Head DS-5M(Equivalent to that for DS-5Mc) (2) Connecting an External Display (3)Connecting USB Peripheral Equipment (4) Connecting to a PC or a PictBridge-SupportingPrinter with USB Cable (5) Connecting the DS Remote Controller DS-RC (6) Connecting to a Network (7) Connecting External I/O Devices (8) Connecting to a Power Supply Microscope Adjustment (1) Use the Appropriate Filters (2) Adjusting the Field Diaphragm (3) Adjusting the Aperture Diaphragm (4) Adjusting the Focus (5) Adjusting the Lamp Voltage (6) Adjusting the Exposure Time (7) Effects of Ambient Light (8) Effects of Vibration Basic Operations 1.1.1Using the DS-L1Panel Switches 1.1.2Using the DS Remote Controller DS-RC 1.1.3Using a Mouse 1.1.4Using a Keyboard 1.2Notes on Using a CF Card 1.3.1Menu Configuration 1.3.2Method of Displaying a Menu 1.4.1Date and Power-save Settings—AdditionalSettings Date:, Time: Power Save Timer Pointer speed Low/Mid/High E.Zoom Step Capture Function 1.4.2Image File Settings—FileSettings Log File Type Outline Guide to Source (Photographing Mode) Settings Auto change CCD mode 1.4.3Startup Condition Settings—PowerOn Settings 1.4.4Adjusting Monitor Output for an External Monitor 1.4.5Settings for Direct Print 1.4.6Settings for Connecting a Microscope 2.1Adjusting Camera Operation and Exposure for Better Picture Quality—CAMMenu 2.1.1Simple Photography—EasyMenu Photographing procedure (1) Choose scene mode and custom mode (2) Adjust the white balance (3) Adjust the focus (4) Correct exposure (1)Choosing scene mode and custom mode (2) Adjusting the white balance (3) Adjusting the focus (4) Correcting exposure (5) Confirming the image (6) Saving the image 2.1.2Special Photography on the Easy Menu—DF/FLScene Mode Page 2.1.3Fine Adjustment before Photographing—Advn.Menu (1)Choose display mode (2)Choose exposure mode (3)Choose photometry mode (4)Choose tone (5)Adjust the white balance (1)Choosing display mode (2)Choosing exposure mode S_AE: MANU: F_AE: (3)Choosing photometry mode (4) Choosing tone (5) Adjusting the white balance (6) Adjusting the picture quality (7) Confirming exposure information (8) Saving the image (9) Saving photographing conditions 2.1.4Special Photography on Advn. Menu —FluorescentPhotographing or Dark Subject Photographing 2.1.5Special Photography on Advn. Menu – Stroboscope Photographing 2.2.1Saving an Image to a CF Card—RECMenu Comment Overlay print Saving an image Interval photography 2.2.2Reproducing Images from a CF Card—VIEWMenu Using the formatting function CF Card Format (FAT16) Format ” 2.3.1Printing an Image 2.3.2Various Print Related Settings Selecting a source Checking the print range Checking print information Scale 3Measuring Two-pointDistance or Entering Information—TOOLMenu 3.1Outline of the TOOL Menu Configuration of the TOOL menu 3.2What is an Overlay Basic method of overlay operation Displaying or hiding the overlay Erasing the overlay Saving or printing the overlay 3.3.1Free-formDrawing 3.3.2Using the Count Marker (to Count the Number of Points) 3.3.3Entering Textual Comments 3.4.1Performing Calibration and Saving Values < When using the magnification of the objective and relay lenses 3.4.2Calling the Calibration Value 3.4.3Measuring the Distance between Two Points Page 3.5.1Using Crosshairs to Adjust the Subject Position Crosshairs display at power-on 3.5.2Using a Scale to Measure the Subject Size 3.5.3Using XY Scales to Measure the Dimensions of the Subject 3.5.4Creating a Translucent Image and Comparing It to a Live Image 3.5.5Using Dual-WindowDisplay to Compare with the Reference Image 4Using the DS-L1via a Network 4.1Typical Method of Use Using a WEB browser to operate the DS-L1from a PC •Get images from the DS-L1 •Control the DS-L1in a simple way Using FTP commands to operate the DS-L1from a PC •Send and save images to the DS-L1 •Advanced control of the DS-L1 Using Telnet commands to operate the DS-L1from a PC Operating on a PC from the DS-L1 4.2.1Items Needing Confirmation 4.2.2Setting Essential Items—NetworkSettings Internal Server Settings for improved security Limiting logins by User ID and Password settings Limiting connectable IPs Saving logs of connected users (NETLOG function) Telnet commands for logs 4.3.1Setting the WEB Browser 4.3.1(1) When Using Internet Explorer Page 4.3.1(2) When Using Netscape Page 4.3.2Using WEB Screens Opening a WEB screen Using ROOM1 (1) Tabs (2) Stream Window •Method for displaying a live image (3) Control Pad •Download BMP •Pickup JPEG (4) Interval Timer (5) Quality (6)E.Zoom (7)Direction buttons (8)Sample image (9) Location Settings by Telnet commands Using ROOM2 (2) Location (3) Current folder name (4) Folders in the CF card (5) Files in the CF card 4.4.1Using FTP Commands to Get DS-L1Image Files from a PC 4.5Using Telnet Commands to Operate on the DS-L1 Commands 4.6.1Setting the FTP Server Functions of the PC 4.6.1(1) When Using Windows NT Workstation Ver Settings Adding a user Specifying the home directory and enabling files to be written to it Other settings When enabling logon as an “anonymous” user 4.6.1(2) When Using Windows 2000 Professional and XP Professional Adding components Specifying the home directory and enabling write to it Page 4.6.1(3) When Using Mac OS Network settings and confirmation 4.6.2Setting External Servers of the DS-L1—NetworkSettings Connection test Ping test Login test 4.6.3Saving Image Files in the FTP Server 4.6.3(1) Using the CAPTURE Button to Save Image Files 4.6.3(2) Using the CAM Menu to Save Image Files Page 5Using the DS-L1when Connected to a PC by a USB Cable 5.1Recommended Operating Environment Supported PCs Supported OSs About the recording media used by the DS-L1 USB interface boards and hubs USB cables USB host drivers 5.2Connecting the DS-L1and a PC 5.3.1Installing the Device Driver 5.3.2Outline of the Disk Drive How to discriminate between the media drive and the CCD image drive 5.3.3Using the Media Drive 5.3.4Using the CCD Image Drive Confirming the updated date/time of image files 5.3.5Removing the DS-L1from the PC 5.4.1Installing the Device Driver 5.4.2Outline of the Disk Drive 5.4.3Using the Media Drive 5.4.4Using the CCD Image Drive Page 5.4.5Removing the DS-L1from the PC 6Using the Microscope Control 6.1Overview 6.2Menu Overview <Precautions regarding menu display Parts Menu displayed Page Page Page 6.2.1Microscope Control Menu Focus (Adjusts focus) <Using the autofocus function Objective <Changes objectives Optical zoom <Changes optical zooms / / Optical path change <Changes optical paths <Selecting the port to which the camera head is connected (Closed)/ (Open) <Opens or closes the shutter Filter <Changes filter cubes Lamp <Controls lamp <To turn the lamp on <To turn the lamp off <Adjusting the brightness of the lamp <Setting the lamp to the preset voltage (9.0 V) 6.2.2Microscope Control Menu Condenser <Changes condenser module Diascopic illumination aperture diaphragm <Adjusts the diascopic illumination aperture diaphragm Diascopic illumination field diaphragm <Adjusts the diascopic illumination field diaphragm Episcopic illumination field diaphragm <Adjusts the episcopic illumination field diaphragm ND filter <Adjusts the transmission factor of the ND filter Analyzer <Inserting/retracting the analyzer <Inserting the analyzer 6.2.3Microscope Status Display/Microscopy Setup Menu (MIC Info Menu) Objective <Shows magnification and NA value of the objective Stage position (Z axis) <Shows the address along vertical axis Optical zoom <Shows the optical zoom magnification Filter <Shows filter name Observation method <Sets the observation method <To set the microscope for user setup microscopy <To link the observation method with the photographing mode of DS-L 6.3Autofocus Precautions Precautions for samples Precautions for 90i setup Other precautions ♦List of autofocus messages Message Content Care and Maintenance Troubleshooting 2Image Output No image appears Only a crossline image appears on a white screen The image is out of focus The image is too dark or too bright The color is poor The contrast is low 3Photographing and Saving Captured images have low resolution and the overlay information cannot be read Photographing is mistimed even when freezing 4Abnormal Behavior The keyboard or mouse does not work or it operates erratically 5Networking Checking network cable connections Checking the set values Checking for presence in a LAN Browser Cannot access the camera from a browser Cannot control the camera from a Images are overwritten when saved to an FTP server An FTP error results when sent to the FTP server 6USB Page 7Microscope Control from the DS-L1 Technical Specifications Recorded image Recorded image file Image file size JPEG compression 2DS Camera Head DS-5M Image pickup device Frame rate Quantization Sensitivity 3DS Cooled Camera Head DS-5Mc CCD cooling 4DS Remote Controller DS-RC 5DS AC adapter Rated output voltage Storage environment Conforming standards