.CAMERA.

 

VI Basic Operations

 

 

 

 

2

Photographing Images

Features of tones

There are seven kinds of tones. Choose an easy-to-observe tone according to the subject.

TONE1: Wide dynamic range

Use this tone when gradation from darks to brights is required (as for subjects that have highlight reflections).

Recommended example: IC chips, part-mounted circuit board, gears, etc.

TONE2: Weak contrast

Use this tone when you want to weaken the contrast.

Recommended example: Cells and tissue, general subjects

TONE3: Standard contrast

Use this tone for ordinary photographing.

Recommended example: Cells and tissue, general subjects

TONE4: Strong contrast

Use this tone when you want to enhance the contrast. The image will have clearly distinguishable darks and brights.

Recommended example: Cells and tissue, general subjects

TONE5: Linear

There is a linear output proportional to the input from the camera. Use this tone when you want to examine the difference in luminous energy from photographed data. Recommended example: DIC observation, PH observation, etc.

TONE6: Provided for metal tissue

Use this tone when you want to suppress unevenness in bright parts while photographing a high-contrast image.

Recommended example: Metal tissue or ceramics and other materials

TONE7: Enhanced contrast

Use this tone when you want to enhance the contrast during observation. Recommended example: Metal tissue or ceramics and other materials, photographing documents with a lens, etc.

(5) Adjusting the white balance

Before photographing an image you need to adjust the white balance of the camera using a subject that has even whites.

1)When using diascopic illumination for microphotography, photograph a transparent part of the preparation. When using episcopic illumination for microphotograph or when using a lens, adjust the white balance with a white subject.

For fluorescent photography, we recommend that the white balance be adjusted under normal light conditions before photographing an image.

2)Next, adjust the exposure.

It would be ideal if the amount of incident light can be adjusted in such a way that the shutter speed falls within a range of about 1/7.5 to 1/250 seconds. To adjust the amount of incident light, adjust the luminous energy of the light source or the iris diaphragm or use an ND filter.

3)

Press

WB

while in this state, and the white balance will be automatically adjusted.

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