| .CAMERA. |
| VI Basic Operations |
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| 2 | Photographing Images |
There are seven kinds of tones. Choose an
Use this tone when gradation from darks to brights is required (as for subjects that have highlight reflections).
Recommended example: IC chips,
Use this tone when you want to weaken the contrast.
Recommended example: Cells and tissue, general subjects
TONE3: Standard contrastUse this tone for ordinary photographing.
Recommended example: Cells and tissue, general subjects
TONE4: Strong contrastUse this tone when you want to enhance the contrast. The image will have clearly distinguishable darks and brights.
Recommended example: Cells and tissue, general subjects
TONE5: LinearThere is a linear output proportional to the input from the camera. Use this tone when you want to examine the difference in luminous energy from photographed data. Recommended example: DIC observation, PH observation, etc.
TONE6: Provided for metal tissueUse this tone when you want to suppress unevenness in bright parts while photographing a
Recommended example: Metal tissue or ceramics and other materials
TONE7: Enhanced contrastUse this tone when you want to enhance the contrast during observation. Recommended example: Metal tissue or ceramics and other materials, photographing documents with a lens, etc.
(5) Adjusting the white balance
Before photographing an image you need to adjust the white balance of the camera using a subject that has even whites.
1)When using diascopic illumination for microphotography, photograph a transparent part of the preparation. When using episcopic illumination for microphotograph or when using a lens, adjust the white balance with a white subject.
For fluorescent photography, we recommend that the white balance be adjusted under normal light conditions before photographing an image.
2)Next, adjust the exposure.
It would be ideal if the amount of incident light can be adjusted in such a way that the shutter speed falls within a range of about 1/7.5 to 1/250 seconds. To adjust the amount of incident light, adjust the luminous energy of the light source or the iris diaphragm or use an ND filter.
3) | Press | WB | while in this state, and the white balance will be automatically adjusted. |
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