V Microscope Adjustment

(6) Adjusting the Exposure Time

Use an ND filter to adjust the illumination for the microscope to a level that yields a suitable exposure time (generally 1/15 to 1/250 s). You can also adjust the camera sensitivity.

Terms

What is an ND filter?

An ND filter is a filter that affects only the amount of light passed, not the color balance of the light. For example, an "ND2" filter cuts transmitted light in half, while an "ND16" filter reduces transmitted light to 1/16th of actual levels.

(7) Effects of Ambient Light

When capturing a dark specimen with a fluorescent microscope, for example, ambient indoor light may enter the optical path. Accordingly, interior lighting should be darkened for photomicrography. Place caps on the binocular eyepiece to prevent light from entering through the eyepiece.

(8) Effects of Vibration

Because photomicrography involves extremely high image resolution, the slightest vibration can affect image quality. To insulate the microscope from vibration, set it on a solid, stable surface on a solid floor. Using an anti-vibration table compatible with the microscope will further reduce the effects of vibration. While actual microphotography is in progress, be careful to avoid touching the microscope or the desk or stand on which it is positioned.

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