VI Basic Operations

CAMERA

 

 

 

 

2Photographing Images

•To suppress unevenness in bright parts while photographing an image with clearly distinguishable dark and bright areas → TONE6

Recommended example: Metal tissue or ceramics and other materials, photographing documents with a lens, etc.

•To enhance contrast for easy recognition → TONE7

Recommended example: Metal tissue or ceramics and other materials, photographing documents with a lens, etc.

Features of tones

There are seven kinds of tones. Choose an easy-to-observe tone according to the subject.

TONE1: Wide dynamic range

Use this tone when gradation from darks to brights is required (as for subjects that have highlight reflections).

Recommended example: IC chips, part-mounted circuit board, gears, etc.

TONE2: Weak contrast

Use this tone when you want to weaken the contrast.

Recommended example: Cells and tissue, general subjects

TONE3: Standard contrast

Use this tone for ordinary photographing.

Recommended example: Cells and tissue, general subjects

TONE4: Strong contrast

Use this tone when you want to enhance the contrast. The image will have clearly distinguishable darks and brights.

Recommended example: Cells and tissue, general subjects

TONE5: Linear

There is a linear output proportional to the input from the camera. Use this tone when you want to examine the difference in luminous energy from photographed data. Recommended example: DIC observation, PH observation, etc.

TONE6: Provided for metal tissue

Use this tone when you want to suppress unevenness in bright parts while photographing a high-contrast image.

Recommended example: Metal tissue or ceramics and other materials

TONE7: Enhanced contrast

Use this tone when you want to enhance the contrast during observation. Recommended example: Metal tissue or ceramics and other materials, photographing documents with a lens, etc.

(5) Adjusting the white balance

Before photographing an image you need to adjust the white balance of the camera using a subject that has even whites.

1)When using diascopic illumination for microphotography, photograph a transparent part of the preparation. When using episcopic illumination for microphotograph or when using a lens, adjust the white balance with a white subject.

For fluorescent photography, we recommend that the white balance be adjusted under normal light conditions before photographing an image.

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