Omega Engineering 20 Programming the CYCLER, The Down arrow key, The Decimal Point and Insert key

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The Alpha/Numeric keys

The Down arrow key

The “Down arrow” key is used for scrolling down through the lines on the screen. It is also used to decrease certain fields when editing.

The Decimal Point and Insert key

The “Decimal Point” key is used when editing numeric fields. It is also used as an Insert key during program editing to insert additional steps and other field sets.

The Delete key

The “Delete” key is used during program editing to delete steps and stages.

The Pause key

The “Pause” key is used during program editing to insert a pause after a stage or to insert increment and decrement times and temperatures. It is also used to pause a program when it is running.

The Enter or Accept key

The “Enter” key is used to enter an operation or the edit mode. It is also used to ‘Accept’ a field or program change.

The Alpha/Numeric keys

The “Alpha/Numeric” keys are used to enter numbers and letters during programming. If it is a numeric field then numbers will be inserted; if it is an alpha field then letters will be inserted. Each key will cycle through the letters shown on the key (0 will insert a space). To insert a number, press and hold the appropriate key.

6. Programming the CYCLER

The table below illustrates the symbols which appear on the LCD display and the relevant keypad button to which they refer.

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Contents Thermal Cyclers TCY 20, 25, 30User’sGuide Shop online atInternet e-mail info@omega.com OMEGAnet Online Service omega.comServicing North America For immediate technical or application assistanceContents 1. Introduction 2. Safety and installationOperator Safety Replacement cable InstallationUnpacking ELECTRICIANWorking Conditions Temperature 3. Cycler TCY SpecificationHeating/Cooling Rate Heated LidDimensions 4. User operation and consumablesPower Heated lid0.5ml Tubes ProgramsMethods00 TCYTCto select Information Configuration5. Front panel controls ConfigurationPrograms MethodsThe End, Finish or Exit key The keysThe Up arrow key The Down arrow keyThe Alpha/Numeric keys 6. Programming the CYCLERStage MethodsPrograms00 Information ConfigurationCycle StepSTEP 2 STEP TEMPLATERun ProgramMethod Option 1 Creating programs using templatesTo save the program To give a program a nameSet password to select ProgramsMethods00 Information Configurationto select DONNA 7 Run programmethod To edit a field or step in an existing program10.0 Post hold72.0ºC 5m0010.0ºCºC UNNAMED #To set a password for a selected program 105ºCProgramming Example programsNew*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Typical 3-Step PCRTo delete a step To insert additional stepsto edit the step as described abovestage to insertTo add a new stage before an existing one To add a new stage at the endThe prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’ To insert a ‘pause’ into a programTemperature profile of incremented Hold Time at step 3 of each cycle To program increment or decrement temperature or timePress “Enter” Press “Pause” to go into edit modetwice to go into edit GEORGE Run ProgrammethodView methodProgram Stopping what you are doingNewprogramMethods Run methodProgramto select To run a program58.0ºC SampleHold @ 72.0ºC7. Information 8. ConfigurationBlock s/nid Unit s/nxxxx ProgramSettingsdefaultsPasswordsFormat memory Settingswill delete ThisSURE ? Passwords9. Notes on transferring protocols from other thermal cyclers Cleaning your CYCLER 10. User maintenanceHeater Block removal Underside of the CYCLER TCY11. Some frequently asked questions Fault Finding 12. Additional informationInsulation testing FusesRETURN REQUESTS/INQUIRIES WARRANTY/DISCLAIMERPRESSURE, STRAIN AND FORCE TEMPERATUREFLOW/LEVEL pH/CONDUCTIVITY