Omega Engineering 48 User operation and consumables, Heated lid, Tubes or reaction vessels, Power

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Dimensions

Dimensions

Height

185mm

Width

185mm

Length

330mm

Power

Power consumption

230W

Units marked 100V will work at any voltage between 90 and 110V.

The performance will vary and will not necessarily meet the above typical specification at the extremes of voltage.

4. User operation and consumables

Heated lid

To release the heated lid, lift the lid catch and the lid will open. To close the lid, push down gently until the catch clicks in place. Do not close the lid without tubes being fitted in the block.

Tubes or reaction vessels

Omega Engineering, Inc. does not recommend any specific tube or reaction vessel other than those described in this manual. We recommend using reaction volumes between 20 and 200µl. The tubes must withstand a pressure of 1 atmosphere at 100°C. Any vessel must be able to withstand the temperatures you are using without any danger of them deforming to the point where they fracture.

To test your tubes, put 25µl of water into each of 5 tubes and subject them to a typical thermal cycling protocol. At the end of the program, measure the volume remaining using a micropipette. A loss of more than 1 or 2µl indicates a vapour leak.

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Contents TCY 20, 25, 30 Thermal CyclersUser’sGuide Shop online atOMEGAnet Online Service omega.com Internet e-mail info@omega.comServicing North America For immediate technical or application assistanceContents Operator Safety 2. Safety and installation1. Introduction Installation Replacement cableUnpacking ELECTRICIANWorking Conditions 3. Cycler TCY Specification TemperatureHeating/Cooling Rate Heated Lid4. User operation and consumables DimensionsPower Heated lid0.5ml Tubes TCYTC ProgramsMethods00to select Information ConfigurationConfiguration 5. Front panel controlsPrograms MethodsThe keys The End, Finish or Exit keyThe Up arrow key The Down arrow key6. Programming the CYCLER The Alpha/Numeric keysMethodsPrograms00 Information Configuration StageCycle Step2 STEP TEMPLATE STEPRun ProgramMethod Option 1 Creating programs using templatesTo give a program a name To save the programProgramsMethods00 Information Configuration Set password to selectto select DONNA 7 Run programmethod To edit a field or step in an existing programPost hold 10.072.0ºC 5m00UNNAMED # 10.0ºCºCTo set a password for a selected program 105ºCExample programs ProgrammingNew*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Typical 3-Step PCRTo insert additional steps To delete a stepto edit the step as described aboveto insert stageTo add a new stage before an existing one To add a new stage at the endTo insert a ‘pause’ into a program The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’To program increment or decrement temperature or time Temperature profile of incremented Hold Time at step 3 of each cyclePress “Enter” Press “Pause” to go into edit modetwice to go into edit Run Programmethod GEORGEView methodProgram Stopping what you are doingRun methodProgram NewprogramMethodsto select To run a programSample 58.0ºCHold @ 72.0ºC8. Configuration 7. InformationBlock s/nid Unit s/nProgramSettingsdefaults xxxxPasswordsFormat memory SettingsThis will deleteSURE ? Passwords9. Notes on transferring protocols from other thermal cyclers 10. User maintenance Cleaning your CYCLERHeater Block removal Underside of the CYCLER TCY11. Some frequently asked questions 12. Additional information Fault FindingInsulation testing FusesWARRANTY/DISCLAIMER RETURN REQUESTS/INQUIRIESTEMPERATURE PRESSURE, STRAIN AND FORCEFLOW/LEVEL pH/CONDUCTIVITY