Omega Engineering 25 Step Template, Run ProgramMethod, to select, STEP TEMPLATE2STEP, Stemplate

Programming Options

There are two options available for creating programs on the CYCLER:

1.Copy and edit one of the pre-written templates.

2.Create a new program from the defaults.

Option 1: Creating programs using templates

There are two pre-written basic PCR templates: a two-step and a three-step template. These can be copied and modified as required. "In denat" stands for Initial Denaturing.

2 STEP2STEMPLATE				3 STEP3 STEMPLATE
Heated Lid			105ºC	Heated Lid			105ºC
Preheat Lid			on	Preheat Lid			on
Pause			off	Pause			off
- - - - - - - - - - -				- - - - - - - - - - -
In denat		94.0ºC 5m00		In denat		94.0ºC 5m00
Hot Start			off	Hot Start			off
- - - Stage 1 - - - -				- - - Stage 1 - - - -
Number cycles			30	Number cycles			30
Step	94.0ºC		0m30 max	Step	94.0ºC		0m30 max
Step	68.0ºC		1m00 max	Step	55.0ºC		0m30 max
- - - - - - - - - - -				Step	72.0ºC		0m30 max
PostFina	runExtn	72.0ºC 5m00		- - - - - - - - - - -
PostFinalhold			1010.0.0ºC	PFostinal rEunxtn 72.0ºC 5m00
				PFostinalhold			1010..00ºC

Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and an RT-PCR template.

*-*New*New- progr a

2STEP TEMPLATE 501[2]

3	STEP	3
[	,	to select ]

2 STEP TEMPLATE2STEP [501]2]

Run ProgramMethod

View MethodProgram

[ , to select ]

Use the “Down Arrow” key to select an existing ‘Template’ program from the menu, e.g. ‘2 Step Template’ or ‘3 Step Template’.

Press the “Enter” key to accept it.

The Template is already defined and can be run as it is or easily copied, modified and saved under a new name.

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