Omega Engineering 25, 48, 20, 30 Unnamed #, 10.0ºCºC, 105ºC, 94.0ºC 5m00, 72.0ºC, PostFin extnru

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To copy a selected program

To copy a selected program

If you select ‘Copy program’ a screen similar to this will appear and the software will automatically give the program the next number until it is named.

Press “Enter” and type in a new name. You will then be able to edit the program as previously described.

To delete a selected program

If you select ‘Delete program’ a screen similar to this will appear.

Press “Enter” to delete the program or “End” to return to the program screen.

To set a password for a selected program

If you want to password protect the program so that other users cannot edit it, select ‘Set password’. A screen similar to this will appear.

Type in the password you wish to give it and press “Enter” . The program list will then display the key symbol next to the name to show that it is locked.

Other users will still be able to copy/view your program but not edit it.

Option 2: Creating a new program

A second option is to create a completely new program without using templates.

UNNAMED #		105ºC
Heated lid
Preheat lid		off
Pause		off
- - - - - - - - - - -
In denat	94.0ºC 5m00
Hot Start		off
- - - - - - - - - - -
PostFin extnru	72.0ºC	5m00
PostFin hold	10.0ºCºC

A number of typical values are pre-entered including:

Heated lid temperature Preheat lid

Initial denaturation Hot start

Final extension Final hold

These values can be edited and the additional stages and steps required for cycling can be added by following the instructions detailed on the following pages.

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Contents Shop online at TCY 20, 25, 30Thermal Cyclers User’sGuideFor immediate technical or application assistance OMEGAnet Online Service omega.comInternet e-mail info@omega.com Servicing North AmericaContents 1. Introduction 2. Safety and installationOperator Safety ELECTRICIAN InstallationReplacement cable UnpackingWorking Conditions Heated Lid 3. Cycler TCY SpecificationTemperature Heating/Cooling RateHeated lid 4. User operation and consumablesDimensions Power0.5ml Tubes Information Configuration TCYTCProgramsMethods00 to selectMethods Configuration5. Front panel controls ProgramsThe Down arrow key The keysThe End, Finish or Exit key The Up arrow keyThe Alpha/Numeric keys 6. Programming the CYCLERStep MethodsPrograms00 Information ConfigurationStage CycleOption 1 Creating programs using templates 2 STEP TEMPLATESTEP Run ProgramMethodTo save the program To give a program a nameTo edit a field or step in an existing program ProgramsMethods00 Information ConfigurationSet password to select to select DONNA 7 Run programmethod5m00 Post hold10.0 72.0ºC105ºC UNNAMED #10.0ºCºC To set a password for a selected programTypical 3-Step PCR Example programsProgramming New*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATEdescribed above To insert additional stepsTo delete a step to edit the step asTo add a new stage at the end to insertstage To add a new stage before an existing oneThe prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’ To insert a ‘pause’ into a programto go into edit mode To program increment or decrement temperature or timeTemperature profile of incremented Hold Time at step 3 of each cycle Press “Enter” Press “Pause”twice to go into edit Stopping what you are doing Run ProgrammethodGEORGE View methodProgramTo run a program Run methodProgramNewprogramMethods to select72.0ºC Sample58.0ºC Hold @Unit s/n 8. Configuration7. Information Block s/nidSettings ProgramSettingsdefaultsxxxx PasswordsFormat memoryPasswords Thiswill delete SURE ?9. Notes on transferring protocols from other thermal cyclers Underside of the CYCLER TCY 10. User maintenanceCleaning your CYCLER Heater Block removal11. Some frequently asked questions Fuses 12. Additional informationFault Finding Insulation testingRETURN REQUESTS/INQUIRIES WARRANTY/DISCLAIMERpH/CONDUCTIVITY TEMPERATUREPRESSURE, STRAIN AND FORCE FLOW/LEVEL