Omega Engineering 20, 48, 30, 25 manual 0.5ml Tubes

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The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 20µl or less.

During the final cool-down, a ring of condensation may form above the liquid level but below the top of the sample block. This is normal and does not effect the reaction.

0.5ml Tubes

The heated lid mechanism of the Cycler TCY with the 0.5ml block is optimised for flat top tubes. In order to fit the maximum number of tubes in the block, they need to be placed so that all the hinges are in the same relative orientation. An example is illustrated below.

Place the tubes in the block in the same orientation in order to achieve the maximum capacity.

Always make sure the pressure on the heated lid is balanced by spreading the tubes across the block or inserting some dummy tubes if necessary.

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Contents Thermal Cyclers TCY 20, 25, 30User’sGuide Shop online atInternet e-mail info@omega.com OMEGAnet Online Service omega.comServicing North America For immediate technical or application assistanceContents 2. Safety and installation 1. IntroductionOperator Safety Replacement cable InstallationUnpacking ELECTRICIANWorking Conditions Temperature 3. Cycler TCY SpecificationHeating/Cooling Rate Heated LidDimensions 4. User operation and consumablesPower Heated lid0.5ml Tubes ProgramsMethods00 TCYTCto select Information Configuration5. Front panel controls ConfigurationPrograms MethodsThe End, Finish or Exit key The keysThe Up arrow key The Down arrow keyThe Alpha/Numeric keys 6. Programming the CYCLERStage MethodsPrograms00 Information ConfigurationCycle StepSTEP 2 STEP TEMPLATERun ProgramMethod Option 1 Creating programs using templatesTo save the program To give a program a nameSet password to select ProgramsMethods00 Information Configurationto select DONNA 7 Run programmethod To edit a field or step in an existing program10.0 Post hold72.0ºC 5m0010.0ºCºC UNNAMED #To set a password for a selected program 105ºCProgramming Example programsNew*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Typical 3-Step PCRTo delete a step To insert additional stepsto edit the step as described abovestage to insertTo add a new stage before an existing one To add a new stage at the endThe prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’ To insert a ‘pause’ into a programTemperature profile of incremented Hold Time at step 3 of each cycle To program increment or decrement temperature or timePress “Enter” Press “Pause” to go into edit modetwice to go into edit GEORGE Run ProgrammethodView methodProgram Stopping what you are doingNewprogramMethods Run methodProgramto select To run a program58.0ºC SampleHold @ 72.0ºC7. Information 8. ConfigurationBlock s/nid Unit s/nxxxx ProgramSettingsdefaultsPasswordsFormat memory Settingswill delete ThisSURE ? Passwords9. Notes on transferring protocols from other thermal cyclers Cleaning your CYCLER 10. User maintenanceHeater Block removal Underside of the CYCLER TCY11. Some frequently asked questions Fault Finding 12. Additional informationInsulation testing FusesRETURN REQUESTS/INQUIRIES WARRANTY/DISCLAIMERPRESSURE, STRAIN AND FORCE TEMPERATUREFLOW/LEVEL pH/CONDUCTIVITY