Omega Engineering 25, 48, 20, 30 manual Contents

Page 3

Contents

 

1.

Introduction

4

2.

Safety and installation

4

3.

Cycler TCY specification

7

4. User operation and consumables

8

 

Heated lid

8

 

Tubes or reaction vessels

8

 

0.5ml Tubes

9

 

Switching on

10

5.

Front panel controls

11

 

The LCD Display

11

 

Indicators

11

 

The keys

12

6. Programming the Cycler TCY

13

 

Programming Options

15

 

Option 1: Creating programs using templates

19

 

Option 2: Creating a new program

20

 

Example programs

20

 

Programming

20

 

To program increment or decrement temperature or time

24

 

To run a program

27

7.

Information

29

8.

Configuration

29

9.

Notes on transferring protocols from other thermal cyclers

32

10. User maintenance

33

 

Cleaning your Cycler TCY

33

 

Heater Block removal

33

11. Some frequently asked questions

34

12. Additional information

35

 

Fault Finding

35

 

Fuses

35

 

The heated lid over-temperature cut-out

35

 

Insulation testing

35

 

Interchangeable blocks

35

3

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Contents Shop online at TCY 20, 25, 30Thermal Cyclers User’sGuideFor immediate technical or application assistance OMEGAnet Online Service omega.comInternet e-mail info@omega.com Servicing North AmericaContents 2. Safety and installation 1. IntroductionOperator Safety ELECTRICIAN InstallationReplacement cable UnpackingWorking Conditions Heated Lid 3. Cycler TCY SpecificationTemperature Heating/Cooling RateHeated lid 4. User operation and consumablesDimensions Power0.5ml Tubes Information Configuration TCYTCProgramsMethods00 to selectMethods Configuration5. Front panel controls ProgramsThe Down arrow key The keysThe End, Finish or Exit key The Up arrow keyThe Alpha/Numeric keys 6. Programming the CYCLERStep MethodsPrograms00 Information ConfigurationStage CycleOption 1 Creating programs using templates 2 STEP TEMPLATESTEP Run ProgramMethodTo save the program To give a program a nameTo edit a field or step in an existing program ProgramsMethods00 Information ConfigurationSet password to select to select DONNA 7 Run programmethod5m00 Post hold10.0 72.0ºC105ºC UNNAMED #10.0ºCºC To set a password for a selected programTypical 3-Step PCR Example programsProgramming New*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATEdescribed above To insert additional stepsTo delete a step to edit the step asTo add a new stage at the end to insertstage To add a new stage before an existing oneThe prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’ To insert a ‘pause’ into a programto go into edit mode To program increment or decrement temperature or timeTemperature profile of incremented Hold Time at step 3 of each cycle Press “Enter” Press “Pause”twice to go into edit Stopping what you are doing Run ProgrammethodGEORGE View methodProgramTo run a program Run methodProgramNewprogramMethods to select72.0ºC Sample58.0ºC Hold @Unit s/n 8. Configuration7. Information Block s/nidSettings ProgramSettingsdefaultsxxxx PasswordsFormat memoryPasswords Thiswill delete SURE ?9. Notes on transferring protocols from other thermal cyclers Underside of the CYCLER TCY 10. User maintenanceCleaning your CYCLER Heater Block removal11. Some frequently asked questions Fuses 12. Additional informationFault Finding Insulation testingRETURN REQUESTS/INQUIRIES WARRANTY/DISCLAIMERpH/CONDUCTIVITY TEMPERATUREPRESSURE, STRAIN AND FORCE FLOW/LEVEL