Omega Engineering 48, 20, 30, 25 manual to select, Example programs, Programming, Typical 3-Step PCR

Contents TCY 20, 25, 30 Thermal CyclersUser’sGuide Shop online atOMEGAnet Online Service omega.com Internet e-mail info@omega.comServicing North America For immediate technical or application assistanceContents Operator Safety 2. Safety and installation1. Introduction Installation Replacement cableUnpacking ELECTRICIANWorking Conditions 3. Cycler TCY Specification TemperatureHeating/Cooling Rate Heated Lid4. User operation and consumables DimensionsPower Heated lid0.5ml Tubes TCYTC ProgramsMethods00to select Information ConfigurationConfiguration 5. Front panel controlsPrograms MethodsThe keys The End, Finish or Exit keyThe Up arrow key The Down arrow key6. Programming the CYCLER The Alpha/Numeric keysMethodsPrograms00 Information Configuration StageCycle Step2 STEP TEMPLATE STEPRun ProgramMethod Option 1 Creating programs using templatesTo give a program a name To save the programProgramsMethods00 Information Configuration Set password to selectto select DONNA 7 Run programmethod To edit a field or step in an existing programPost hold 10.072.0ºC 5m00UNNAMED # 10.0ºCºCTo set a password for a selected program 105ºCExample programs ProgrammingNew*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Typical 3-Step PCRTo insert additional steps To delete a stepto edit the step as described aboveto insert stageTo add a new stage before an existing one To add a new stage at the endTo insert a ‘pause’ into a program The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’To program increment or decrement temperature or time Temperature profile of incremented Hold Time at step 3 of each cyclePress “Enter” Press “Pause” to go into edit modetwice to go into edit Run Programmethod GEORGEView methodProgram Stopping what you are doingRun methodProgram NewprogramMethodsto select To run a programSample 58.0ºCHold @ 72.0ºC8. Configuration 7. InformationBlock s/nid Unit s/nProgramSettingsdefaults xxxxPasswordsFormat memory SettingsThis will deleteSURE ? Passwords9. Notes on transferring protocols from other thermal cyclers 10. User maintenance Cleaning your CYCLERHeater Block removal Underside of the CYCLER TCY11. Some frequently asked questions 12. Additional information Fault FindingInsulation testing FusesWARRANTY/DISCLAIMER RETURN REQUESTS/INQUIRIESTEMPERATURE PRESSURE, STRAIN AND FORCEFLOW/LEVEL pH/CONDUCTIVITY