Omega Engineering 48, 20, 30, 25 manual to select, Example programs, Programming, Typical 3-Step PCR

Example programs

A couple of example thermal cycling programs are shown below.

Typical 3-Step PCR				3-Step PCR with multiple ‘Stages’
Heated Lid			105	Heated Lid			105
Preheat Lid			on	Preheat Lid			on
Pause			off	Pause			off
- - - - - - - - - - - - - - - -				- - - - - - - - - - - - - - - -
In denat	94°c	5min	off	In denat	94°c	5min	off
Hot Start		- - - - - -		Hot Start		- - - - - -
- - - - - - Stage 1			30	- - - - - - Stage 1			10
Number Cycles		0m30		Number Cycles		0m30
Step	94°c		max	step	94°c		max
Step	55°c	0m30	max	step	60°c	0m30	max
step	72°c	0m30	max	step	72°c	0m30	max
- - - - - - - - - - - - - - - -
				- - - - - - Stage 2		- - - - - -
Post run		72°c	5m
				Number Cycles			20
Post hold		4ºC	10m
				step	94°c	0m30	max
				step	55°c	0m30	max
				step	72°c	0m30	max
				- - - - - - - - - - - - - - - -			5m
				Post run		72°c
				Post hold		4ºC	10m

Programming

-**New*New- program ** []] 2 STEP TEMPLATE 5012 3 STEP TEMPLATE 5023

[ , to select ]

Create a new program by selecting

-* New *- from the menu and press “Enter”

Give the program a name and edit any of the default fields as described above.

To add a stage and then edit it

Use the “Down Arrow” key to scroll to the dotted line below ‘Hot Start’; it will start to flash.

Press "Enter"

to insert a stage

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