Omega Engineering 30, 48, 20, 25 manual Working Conditions

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Working Conditions

The thermal cycler is designed operate under the following conditions:

∙Indoor use

∙Ambient temperature range +5oC to +40oC

∙Altitude to 2000m

∙Relative humidity not exceeding 95%

∙Power supply fluctuations not exceeding 10%

∙Over voltage category II IEC 60364-4-443

∙Pollution degree 2

Note: The control specifications are quoted at an ambient temperature of 20oC. The specification may deteriorate outside an ambient temperature of 10oC to 30oC.

The instrument has been tested for radio frequency interference and is certified under EN61326.

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Contents User’sGuide TCY 20, 25, 30Thermal Cyclers Shop online atServicing North America OMEGAnet Online Service omega.comInternet e-mail info@omega.com For immediate technical or application assistanceContents 2. Safety and installation 1. IntroductionOperator Safety Unpacking InstallationReplacement cable ELECTRICIANWorking Conditions Heating/Cooling Rate 3. Cycler TCY SpecificationTemperature Heated LidPower 4. User operation and consumablesDimensions Heated lid0.5ml Tubes to select TCYTCProgramsMethods00 Information ConfigurationPrograms Configuration5. Front panel controls MethodsThe Up arrow key The keysThe End, Finish or Exit key The Down arrow key6. Programming the CYCLER The Alpha/Numeric keysCycle MethodsPrograms00 Information ConfigurationStage StepRun ProgramMethod 2 STEP TEMPLATESTEP Option 1 Creating programs using templatesTo give a program a name To save the programto select DONNA 7 Run programmethod ProgramsMethods00 Information ConfigurationSet password to select To edit a field or step in an existing program72.0ºC Post hold10.0 5m00To set a password for a selected program UNNAMED #10.0ºCºC 105ºCNew*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Example programsProgramming Typical 3-Step PCRto edit the step as To insert additional stepsTo delete a step described aboveTo add a new stage before an existing one to insertstage To add a new stage at the endTo insert a ‘pause’ into a program The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’Press “Enter” Press “Pause” To program increment or decrement temperature or timeTemperature profile of incremented Hold Time at step 3 of each cycle to go into edit modetwice to go into edit View methodProgram Run ProgrammethodGEORGE Stopping what you are doingto select Run methodProgramNewprogramMethods To run a programHold @ Sample58.0ºC 72.0ºCBlock s/nid 8. Configuration7. Information Unit s/nPasswordsFormat memory ProgramSettingsdefaultsxxxx SettingsSURE ? Thiswill delete Passwords9. Notes on transferring protocols from other thermal cyclers Heater Block removal 10. User maintenanceCleaning your CYCLER Underside of the CYCLER TCY11. Some frequently asked questions Insulation testing 12. Additional informationFault Finding FusesWARRANTY/DISCLAIMER RETURN REQUESTS/INQUIRIESFLOW/LEVEL TEMPERATUREPRESSURE, STRAIN AND FORCE pH/CONDUCTIVITY