Omega Engineering 48, 20, 30, 25 manual 58.0ºC, Hold @, 72.0ºC, Sample 72.0º C, Sample 94.0ºC

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Sample

Cycle 4/30				56m08
Sample	58.0ºC		5.7 /S
Hold @	58.0ºC		00m17
[	to STOP			]
[	to	PAUSE		]
[	for	info		]

During the run the LCD screen will report:

1.The cycle number

2.The time remaining (counting down in seconds)

3.The sample temperature (whether holding or ramping)

4.Maximal ramp rate

5.The current step of the run

6.A count down of the hold time.

An example is shown here.

Manual pause

Cycle 24/30			19m26
Sample		72.0ºC	2.7 /S
Hold	@	72.0ºC	00m10
[		PAUSE ?	NO	]
[	YES or			]

Cycle 24/30		19m26
Sample 72.0ºC		5.7 /S
Paused	to STOP	]
[
[	to CONTINUE ]
[	for info	]

To pause a program while it is running, press “Pause”

The run will continue but the prompt line will alternate ‘PAUSE?’ and ‘YES or NO’

To confirm that you want the program to pause press “Enter”

If you have pressed the “Pause” key by mistake and you want the program to continue, press “End”

The unit will emit a beeping sound while paused.

To resume the run, press “Pause ”

followed by “Enter” to continue.

Manual Stop

Cycle 28/30			10m39
Sample 94.0ºC			5.7 /S
Hold	@ 94.0ºC		00m10
[	STOP	PROGRAM?		]
[	YES	or	NO	]

To stop a program while it is running, press “End”

The run will continue but the prompt line will alternate ‘STOP PROGRAM?’ and ‘YES or NO’

To confirm that you want to stop the program press “Enter”

If you have pressed the “End” key by mistake and you want the program to continue, press “End” again.

When a program is stopped the unit will emit a beeping sound and the original program screen will appear.

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Contents TCY 20, 25, 30 Thermal CyclersUser’sGuide Shop online atOMEGAnet Online Service omega.com Internet e-mail info@omega.comServicing North America For immediate technical or application assistanceContents 1. Introduction 2. Safety and installationOperator Safety Installation Replacement cableUnpacking ELECTRICIANWorking Conditions 3. Cycler TCY Specification TemperatureHeating/Cooling Rate Heated Lid4. User operation and consumables DimensionsPower Heated lid0.5ml Tubes TCYTC ProgramsMethods00to select Information ConfigurationConfiguration 5. Front panel controlsPrograms MethodsThe keys The End, Finish or Exit keyThe Up arrow key The Down arrow key6. Programming the CYCLER The Alpha/Numeric keysMethodsPrograms00 Information Configuration StageCycle Step2 STEP TEMPLATE STEPRun ProgramMethod Option 1 Creating programs using templatesTo give a program a name To save the programProgramsMethods00 Information Configuration Set password to selectto select DONNA 7 Run programmethod To edit a field or step in an existing programPost hold 10.072.0ºC 5m00UNNAMED # 10.0ºCºCTo set a password for a selected program 105ºCExample programs ProgrammingNew*New- program ** 2 STEP TEMPLATE 5012 3 STEP TEMPLATE Typical 3-Step PCRTo insert additional steps To delete a stepto edit the step as described aboveto insert stageTo add a new stage before an existing one To add a new stage at the endTo insert a ‘pause’ into a program The prompt line will flash between ‘CHANGE TO PAUSE?’ and ‘YES or NO’To program increment or decrement temperature or time Temperature profile of incremented Hold Time at step 3 of each cyclePress “Enter” Press “Pause” to go into edit modetwice to go into edit Run Programmethod GEORGEView methodProgram Stopping what you are doingRun methodProgram NewprogramMethodsto select To run a programSample 58.0ºCHold @ 72.0ºC8. Configuration 7. InformationBlock s/nid Unit s/nProgramSettingsdefaults xxxxPasswordsFormat memory SettingsThis will deleteSURE ? Passwords9. Notes on transferring protocols from other thermal cyclers 10. User maintenance Cleaning your CYCLERHeater Block removal Underside of the CYCLER TCY11. Some frequently asked questions 12. Additional information Fault FindingInsulation testing FusesWARRANTY/DISCLAIMER RETURN REQUESTS/INQUIRIESTEMPERATURE PRESSURE, STRAIN AND FORCEFLOW/LEVEL pH/CONDUCTIVITY